Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11970 | 3' | -56.2 | NC_003278.1 | + | 21625 | 0.74 | 0.180289 |
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Target: 5'- aGGGCGGgA--GCCUcgggaaacuccccgUCGAGGCCGGCGa -3' miRNA: 3'- -CCUGCCgUagUGGA--------------AGCUCCGGCUGU- -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 21759 | 0.73 | 0.225898 |
|
Target: 5'- cGGCGGCGacacuuacuacaUCACCaUCcAGGCCGGCGc -3' miRNA: 3'- cCUGCCGU------------AGUGGaAGcUCCGGCUGU- -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 23014 | 0.68 | 0.441906 |
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Target: 5'- aGGAUGGCGaccagCACCgcuaccUGcAGGCCGACc -3' miRNA: 3'- -CCUGCCGUa----GUGGaa----GC-UCCGGCUGu -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 28257 | 0.7 | 0.308689 |
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Target: 5'- aGGCGGCGgguuCCUugUCGGuGGCCGGCGa -3' miRNA: 3'- cCUGCCGUagu-GGA--AGCU-CCGGCUGU- -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 30986 | 0.7 | 0.329426 |
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Target: 5'- cGGACaGGCAcgCAUCgaacagcgcugggUCGAGGCCGcGCAg -3' miRNA: 3'- -CCUG-CCGUa-GUGGa------------AGCUCCGGC-UGU- -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 32373 | 0.68 | 0.412718 |
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Target: 5'- gGGAUGGCA-CACggugcaUCG-GGCCGGCGg -3' miRNA: 3'- -CCUGCCGUaGUGga----AGCuCCGGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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