Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11970 | 3' | -56.2 | NC_003278.1 | + | 2552 | 0.68 | 0.412718 |
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Target: 5'- aGAuCGGCGUgGCCgguguugUCGuugcGGCCGGCGg -3' miRNA: 3'- cCU-GCCGUAgUGGa------AGCu---CCGGCUGU- -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 23014 | 0.68 | 0.441906 |
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Target: 5'- aGGAUGGCGaccagCACCgcuaccUGcAGGCCGACc -3' miRNA: 3'- -CCUGCCGUa----GUGGaa----GC-UCCGGCUGu -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 9533 | 0.67 | 0.493015 |
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Target: 5'- aGGcCGGCAccaUCacgGCCcUCGAGGCCGcCc -3' miRNA: 3'- -CCuGCCGU---AG---UGGaAGCUCCGGCuGu -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 14229 | 0.67 | 0.514212 |
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Target: 5'- cGGCGGCG-CGCCcggCGAgacGGCUGACc -3' miRNA: 3'- cCUGCCGUaGUGGaa-GCU---CCGGCUGu -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 14154 | 0.66 | 0.531433 |
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Target: 5'- cGGcGCGGCAgCGCUgaucgcggcgagCGAGGCCGGgAa -3' miRNA: 3'- -CC-UGCCGUaGUGGaa----------GCUCCGGCUgU- -5' |
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| 11970 | 3' | -56.2 | NC_003278.1 | + | 10176 | 1.11 | 0.000387 |
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Target: 5'- aGGACGGCAUCACCUUCGAGGCCGACAu -3' miRNA: 3'- -CCUGCCGUAGUGGAAGCUCCGGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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