Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11971 | 3' | -55.8 | NC_003278.1 | + | 6471 | 0.66 | 0.526177 |
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Target: 5'- aUUUcCGGCGUgcugcaGGCCGGCAaGGGCGCc -3' miRNA: 3'- -AGAuGCCGCG------UCGGUUGUcUUCGCGc -5' |
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| 11971 | 3' | -55.8 | NC_003278.1 | + | 16737 | 0.67 | 0.504438 |
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Target: 5'- gCUGCaGGCGUAccgccGCCAACu---GCGCGa -3' miRNA: 3'- aGAUG-CCGCGU-----CGGUUGucuuCGCGC- -5' |
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| 11971 | 3' | -55.8 | NC_003278.1 | + | 14783 | 0.67 | 0.501211 |
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Target: 5'- gCUACGGCaucacggacgcacuGCAuGCCAGC-GAGGCGg- -3' miRNA: 3'- aGAUGCCG--------------CGU-CGGUUGuCUUCGCgc -5' |
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| 11971 | 3' | -55.8 | NC_003278.1 | + | 1481 | 0.67 | 0.493715 |
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Target: 5'- gCUGCaGGCGC-GUCuGCAuuGGCGCGa -3' miRNA: 3'- aGAUG-CCGCGuCGGuUGUcuUCGCGC- -5' |
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| 11971 | 3' | -55.8 | NC_003278.1 | + | 19660 | 0.67 | 0.483097 |
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Target: 5'- --cGCaGCGCuuacgcaaccucAGCCAgcaGCAGGAGCGCc -3' miRNA: 3'- agaUGcCGCG------------UCGGU---UGUCUUCGCGc -5' |
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| 11971 | 3' | -55.8 | NC_003278.1 | + | 32941 | 0.67 | 0.4622 |
|
Target: 5'- aCUACGGCGUAGuuggguucgaCCAAUccgccGGCGCGg -3' miRNA: 3'- aGAUGCCGCGUC----------GGUUGucu--UCGCGC- -5' |
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| 11971 | 3' | -55.8 | NC_003278.1 | + | 10546 | 1.09 | 0.000456 |
|
Target: 5'- aUCUACGGCGCAGCCAACAGAAGCGCGu -3' miRNA: 3'- -AGAUGCCGCGUCGGUUGUCUUCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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