miRNA: 3'- -CGGUCUUGGac-CGGCCGguCCACGag -5'
miRNA: 3'- -CGGUCuUGGaccGGCCGGU-CCACGag -5'
miRNA: 3'- -CGGUCUUGGACCGGCCGGUCCACGAG- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11976 | 3' | -60.8 | NC_003278.1 | + | 2790 | 0.66 | 0.291156 |
|
Target: 5'- cGCCAGAGCCcauGCCGGU--GGUGUc- -3' miRNA: 3'- -CGGUCUUGGac-CGGCCGguCCACGag -5' |
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| 11976 | 3' | -60.8 | NC_003278.1 | + | 32742 | 0.66 | 0.313827 |
|
Target: 5'- gGCCAGuACUa--CCGGCCAgGGUGCa- -3' miRNA: 3'- -CGGUCuUGGaccGGCCGGU-CCACGag -5' |
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| 11976 | 3' | -60.8 | NC_003278.1 | + | 12562 | 1.11 | 0.000096 |
|
Target: 5'- gGCCAGAACCUGGCCGGCCAGGUGCUCa -3' miRNA: 3'- -CGGUCUUGGACCGGCCGGUCCACGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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