miRNA: 3'- -GGUCGC-GuaCUGUUGCUuGaaGCAGC- -5'
miRNA: 3'- gGUCGCGuACUGUUGCU---UGAagCAGc -5'
miRNA: 3'- -GGUCGCGuAC---UGUUGCUugaAGCAGC- -5'
miRNA: 3'- -GGUCGCGUACUGUUGCUUGAAGCAGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11977 | 3' | -49.4 | NC_003278.1 | + | 13320 | 0.7 | 0.740964 |
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Target: 5'- aUCAGCGaCggGGCGACGGuCggCGUCGu -3' miRNA: 3'- -GGUCGC-GuaCUGUUGCUuGaaGCAGC- -5' |
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| 11977 | 3' | -49.4 | NC_003278.1 | + | 10308 | 0.71 | 0.649189 |
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Target: 5'- gCGGCGCuUGACGACGAcacGCUcgGUCa -3' miRNA: 3'- gGUCGCGuACUGUUGCU---UGAagCAGc -5' |
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| 11977 | 3' | -49.4 | NC_003278.1 | + | 7508 | 0.73 | 0.556172 |
|
Target: 5'- cCCAGCGC-UGgucGCGGCGGuagUCGUCGa -3' miRNA: 3'- -GGUCGCGuAC---UGUUGCUugaAGCAGC- -5' |
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| 11977 | 3' | -49.4 | NC_003278.1 | + | 12658 | 1.11 | 0.002061 |
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Target: 5'- gCCAGCGCAUGACAACGAACUUCGUCGc -3' miRNA: 3'- -GGUCGCGUACUGUUGCUUGAAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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