miRNA: 3'- -GCUuGAUGUUGc---AGUUCGCGGa---CCAG- -5'
miRNA: 3'- -GCUuGA--UGUUGcAGUUCGCGGACCaG- -5'
miRNA: 3'- gcUUGaUGUUGCAGU--------UCGCGGACCAg -5'
miRNA: 3'- -GCUUGau------GUUGCAGUUC-GCGGaCCaG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11979 | 5' | -51.3 | NC_003278.1 | + | 29532 | 0.73 | 0.403996 |
|
Target: 5'- gCGAuCUGCAGCuugaUCGAGCGCUgcagGGUCu -3' miRNA: 3'- -GCUuGAUGUUGc---AGUUCGCGGa---CCAG- -5' |
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| 11979 | 5' | -51.3 | NC_003278.1 | + | 31725 | 0.74 | 0.375253 |
|
Target: 5'- cCGGcCUccGCGACuUCGAGCGCCUGGcCg -3' miRNA: 3'- -GCUuGA--UGUUGcAGUUCGCGGACCaG- -5' |
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| 11979 | 5' | -51.3 | NC_003278.1 | + | 32026 | 0.75 | 0.322048 |
|
Target: 5'- -cAGC-GCAGCGUCAacucguucAGCGCCUGGUa -3' miRNA: 3'- gcUUGaUGUUGCAGU--------UCGCGGACCAg -5' |
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| 11979 | 5' | -51.3 | NC_003278.1 | + | 32617 | 0.67 | 0.72794 |
|
Target: 5'- uCGGGCgccaucauCGGCGUCAAGuCGCCcGGcCa -3' miRNA: 3'- -GCUUGau------GUUGCAGUUC-GCGGaCCaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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