Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1198 | 5' | -52.6 | NC_001132.2 | + | 124553 | 0.66 | 0.991035 |
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Target: 5'- --cGGGA-CCCGugGAcaCgUCGUCGGa -3' miRNA: 3'- gaaUCUUgGGGCugCUa-GgAGCAGCC- -5' |
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| 1198 | 5' | -52.6 | NC_001132.2 | + | 127689 | 0.67 | 0.97708 |
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Target: 5'- ----cGACgCCGACGAggaUCGUCGGg -3' miRNA: 3'- gaaucUUGgGGCUGCUaggAGCAGCC- -5' |
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| 1198 | 5' | -52.6 | NC_001132.2 | + | 100551 | 0.67 | 0.974584 |
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Target: 5'- -gUAGuAGCCUCGAUaGGUUCUCGUUGa -3' miRNA: 3'- gaAUC-UUGGGGCUG-CUAGGAGCAGCc -5' |
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| 1198 | 5' | -52.6 | NC_001132.2 | + | 751 | 0.67 | 0.974584 |
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Target: 5'- ---cGGACCUCcAUGGUCgUUCGUCGGg -3' miRNA: 3'- gaauCUUGGGGcUGCUAG-GAGCAGCC- -5' |
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| 1198 | 5' | -52.6 | NC_001132.2 | + | 11413 | 0.67 | 0.974584 |
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Target: 5'- --gAGGACUCCGACGccuUUCUCGcguuaCGGg -3' miRNA: 3'- gaaUCUUGGGGCUGCu--AGGAGCa----GCC- -5' |
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| 1198 | 5' | -52.6 | NC_001132.2 | + | 122744 | 0.68 | 0.971897 |
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Target: 5'- ---uGAuCCCCGGCcuccuucggauGAUCCUCcUCGGg -3' miRNA: 3'- gaauCUuGGGGCUG-----------CUAGGAGcAGCC- -5' |
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| 1198 | 5' | -52.6 | NC_001132.2 | + | 95614 | 0.68 | 0.965919 |
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Target: 5'- -aUGGGACUCCGACGG-CgUCGUUa- -3' miRNA: 3'- gaAUCUUGGGGCUGCUaGgAGCAGcc -5' |
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| 1198 | 5' | -52.6 | NC_001132.2 | + | 73575 | 0.69 | 0.93321 |
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Target: 5'- --aAGAACCCCuACGccguccGUCUUCGUCGu -3' miRNA: 3'- gaaUCUUGGGGcUGC------UAGGAGCAGCc -5' |
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| 1198 | 5' | -52.6 | NC_001132.2 | + | 159732 | 0.72 | 0.856243 |
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Target: 5'- --gAGAACUCCGACGuuuuaaccaCUUCGUUGGg -3' miRNA: 3'- gaaUCUUGGGGCUGCua-------GGAGCAGCC- -5' |
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| 1198 | 5' | -52.6 | NC_001132.2 | + | 127645 | 0.72 | 0.840371 |
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Target: 5'- ---cGGACgCCGACGAggaUCGUCGGg -3' miRNA: 3'- gaauCUUGgGGCUGCUaggAGCAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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