Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11986 | 3' | -54.2 | NC_003278.1 | + | 4468 | 0.66 | 0.70632 |
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Target: 5'- -gUgGUCGCCCCuUCGacgGCGAUgCggaACCa -3' miRNA: 3'- ugAgCAGCGGGG-AGCa--UGCUA-Ga--UGG- -5' |
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| 11986 | 3' | -54.2 | NC_003278.1 | + | 3563 | 0.66 | 0.695235 |
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Target: 5'- ---gGUCGCCCUUCaGUuCGAcacCUACCg -3' miRNA: 3'- ugagCAGCGGGGAG-CAuGCUa--GAUGG- -5' |
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| 11986 | 3' | -54.2 | NC_003278.1 | + | 27163 | 0.66 | 0.672885 |
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Target: 5'- aGCUCa--GCCCCUCGggccuCGAgcgGCCa -3' miRNA: 3'- -UGAGcagCGGGGAGCau---GCUagaUGG- -5' |
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| 11986 | 3' | -54.2 | NC_003278.1 | + | 5453 | 0.66 | 0.672885 |
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Target: 5'- aGCUCGUCGaCgCCguagaCGU-UGAUCUGCUu -3' miRNA: 3'- -UGAGCAGC-GgGGa----GCAuGCUAGAUGG- -5' |
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| 11986 | 3' | -54.2 | NC_003278.1 | + | 10512 | 0.66 | 0.661643 |
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Target: 5'- cCUCGUgcUGCCCUcgcgCGUucgcucgcccGCGAUCUACg -3' miRNA: 3'- uGAGCA--GCGGGGa---GCA----------UGCUAGAUGg -5' |
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| 11986 | 3' | -54.2 | NC_003278.1 | + | 13265 | 0.67 | 0.593969 |
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Target: 5'- gAC-CGUCGCCCCgUCGc-UGAUC-GCCu -3' miRNA: 3'- -UGaGCAGCGGGG-AGCauGCUAGaUGG- -5' |
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| 11986 | 3' | -54.2 | NC_003278.1 | + | 29242 | 0.67 | 0.593969 |
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Target: 5'- uGCUCGgcgugGCCgCCg-GUGCGAUCUucACCg -3' miRNA: 3'- -UGAGCag---CGG-GGagCAUGCUAGA--UGG- -5' |
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| 11986 | 3' | -54.2 | NC_003278.1 | + | 8807 | 0.7 | 0.463955 |
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Target: 5'- cCUCGUCGCCgCCggagcUCGccuCGAcCUGCCg -3' miRNA: 3'- uGAGCAGCGG-GG-----AGCau-GCUaGAUGG- -5' |
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| 11986 | 3' | -54.2 | NC_003278.1 | + | 15196 | 1.11 | 0.000586 |
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Target: 5'- aACUCGUCGCCCCUCGUACGAUCUACCu -3' miRNA: 3'- -UGAGCAGCGGGGAGCAUGCUAGAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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