Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11988 | 3' | -60.8 | NC_003278.1 | + | 14247 | 0.66 | 0.284062 |
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Target: 5'- uAugGGUgGg-CUGCCAcuucUUCCCGgCCUCg -3' miRNA: 3'- -UugCCGgCagGACGGU----AAGGGC-GGAG- -5' |
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| 11988 | 3' | -60.8 | NC_003278.1 | + | 21537 | 0.67 | 0.263043 |
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Target: 5'- --gGGCCGUCagGCUGUcgCCgGCCUCg -3' miRNA: 3'- uugCCGGCAGgaCGGUAa-GGgCGGAG- -5' |
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| 11988 | 3' | -60.8 | NC_003278.1 | + | 4479 | 0.67 | 0.243305 |
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Target: 5'- uGCGGCCGUCCgugGUCg--CCC-CUUCg -3' miRNA: 3'- uUGCCGGCAGGa--CGGuaaGGGcGGAG- -5' |
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| 11988 | 3' | -60.8 | NC_003278.1 | + | 9749 | 0.67 | 0.237004 |
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Target: 5'- gAGCGGCCG-CUUaCCAUcaguggcugUCCCGCCg- -3' miRNA: 3'- -UUGCCGGCaGGAcGGUA---------AGGGCGGag -5' |
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| 11988 | 3' | -60.8 | NC_003278.1 | + | 16842 | 0.67 | 0.230232 |
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Target: 5'- aGAUGG-CGUCUUguagcuucacGCCGUacaagcuUCCCGCCUCg -3' miRNA: 3'- -UUGCCgGCAGGA----------CGGUA-------AGGGCGGAG- -5' |
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| 11988 | 3' | -60.8 | NC_003278.1 | + | 23388 | 0.68 | 0.202029 |
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Target: 5'- --aGGCUGUgCUGCaCAUUgCCGCCg- -3' miRNA: 3'- uugCCGGCAgGACG-GUAAgGGCGGag -5' |
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| 11988 | 3' | -60.8 | NC_003278.1 | + | 21863 | 0.69 | 0.176392 |
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Target: 5'- aGGCGGCgCG-CCUGCgcg-CCCGCCUg -3' miRNA: 3'- -UUGCCG-GCaGGACGguaaGGGCGGAg -5' |
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| 11988 | 3' | -60.8 | NC_003278.1 | + | 31081 | 0.7 | 0.161095 |
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Target: 5'- uGCGGCCcagcgcuugcucagGUCCUGCCAgaucgcgUCgCCGUCUUc -3' miRNA: 3'- uUGCCGG--------------CAGGACGGUa------AG-GGCGGAG- -5' |
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| 11988 | 3' | -60.8 | NC_003278.1 | + | 16261 | 1.07 | 0.000182 |
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Target: 5'- aAACGGCCGUCCUGCCAUUCCCGCCUCa -3' miRNA: 3'- -UUGCCGGCAGGACGGUAAGGGCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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