Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11989 | 5' | -57.4 | NC_003278.1 | + | 31089 | 0.66 | 0.493015 |
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Target: 5'- aGCGUCGCUGCGGCccagcgcuuGCUC-AGGUc -3' miRNA: 3'- gUGCAGUGGCGCUGcu-------CGAGcUCCAa -5' |
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| 11989 | 5' | -57.4 | NC_003278.1 | + | 8884 | 0.66 | 0.462001 |
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Target: 5'- -uCGUCGCCaGCGGC-AGgUCGAGGc- -3' miRNA: 3'- guGCAGUGG-CGCUGcUCgAGCUCCaa -5' |
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| 11989 | 5' | -57.4 | NC_003278.1 | + | 22316 | 0.66 | 0.451893 |
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Target: 5'- -uCGUCGauGCGcuUGAGCUCGAGGg- -3' miRNA: 3'- guGCAGUggCGCu-GCUCGAGCUCCaa -5' |
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| 11989 | 5' | -57.4 | NC_003278.1 | + | 32142 | 0.67 | 0.422315 |
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Target: 5'- uCACG--ACUGCGGCGAGCUgGGcGGUc -3' miRNA: 3'- -GUGCagUGGCGCUGCUCGAgCU-CCAa -5' |
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| 11989 | 5' | -57.4 | NC_003278.1 | + | 11867 | 0.67 | 0.393932 |
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Target: 5'- gCAUGgucCACCGCGGCGAugguGC-CGAGGc- -3' miRNA: 3'- -GUGCa--GUGGCGCUGCU----CGaGCUCCaa -5' |
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| 11989 | 5' | -57.4 | NC_003278.1 | + | 13005 | 0.71 | 0.214083 |
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Target: 5'- gACGUUGuaguUCGCGGCGAGCuggUCGAGGUc -3' miRNA: 3'- gUGCAGU----GGCGCUGCUCG---AGCUCCAa -5' |
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| 11989 | 5' | -57.4 | NC_003278.1 | + | 16675 | 1.05 | 0.00065 |
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Target: 5'- gCACGUCACCGCGACGAGCUCGAGGUUg -3' miRNA: 3'- -GUGCAGUGGCGCUGCUCGAGCUCCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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