Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11994 | 5' | -58.1 | NC_003278.1 | + | 1960 | 0.66 | 0.445529 |
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Target: 5'- cGGaGUGcCG-GAguGGUUCUGCGCCCUa -3' miRNA: 3'- uCC-CGCaGCaCU--UCAGGACGUGGGAc -5' |
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| 11994 | 5' | -58.1 | NC_003278.1 | + | 10234 | 0.66 | 0.397377 |
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Target: 5'- -cGGCcUCGaaggUGAugccGUCCUGCACCUUGg -3' miRNA: 3'- ucCCGcAGC----ACUu---CAGGACGUGGGAC- -5' |
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| 11994 | 5' | -58.1 | NC_003278.1 | + | 32834 | 0.67 | 0.388155 |
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Target: 5'- cAGGGCcacgGUGGcgcgGGUCCgcgacagcaaaUGCACCCUGg -3' miRNA: 3'- -UCCCGcag-CACU----UCAGG-----------ACGUGGGAC- -5' |
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| 11994 | 5' | -58.1 | NC_003278.1 | + | 30843 | 0.67 | 0.364845 |
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Target: 5'- -aGGCGUUucagcgcgGUGAGGccggcuaccucguucUCCUGCugCCUGg -3' miRNA: 3'- ucCCGCAG--------CACUUC---------------AGGACGugGGAC- -5' |
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| 11994 | 5' | -58.1 | NC_003278.1 | + | 4882 | 0.67 | 0.344202 |
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Target: 5'- cAGGGUGUCGgGAuuGGUCUUGCGgCUg- -3' miRNA: 3'- -UCCCGCAGCaCU--UCAGGACGUgGGac -5' |
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| 11994 | 5' | -58.1 | NC_003278.1 | + | 19579 | 0.67 | 0.344202 |
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Target: 5'- cAGGGCGUggcUGUGcugcAGGUgCUGCugCUUGa -3' miRNA: 3'- -UCCCGCA---GCAC----UUCAgGACGugGGAC- -5' |
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| 11994 | 5' | -58.1 | NC_003278.1 | + | 18235 | 1.07 | 0.000384 |
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Target: 5'- cAGGGCGUCGUGAAGUCCUGCACCCUGc -3' miRNA: 3'- -UCCCGCAGCACUUCAGGACGUGGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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