Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11996 | 3' | -50.5 | NC_003278.1 | + | 14164 | 0.66 | 0.822293 |
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Target: 5'- gCgCUGAUCGCGgcGAGcGAgGCCGg- -3' miRNA: 3'- aGgGACUAGCGCauCUU-UUgCGGUac -5' |
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| 11996 | 3' | -50.5 | NC_003278.1 | + | 3924 | 0.67 | 0.791874 |
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Target: 5'- gCCCUGGUaGCGcUGGAuccGCGCC-UGg -3' miRNA: 3'- aGGGACUAgCGC-AUCUuu-UGCGGuAC- -5' |
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| 11996 | 3' | -50.5 | NC_003278.1 | + | 20516 | 0.68 | 0.737558 |
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Target: 5'- aCCCUGAUgCGCGccgucGAGGGCgGCCAg- -3' miRNA: 3'- aGGGACUA-GCGCau---CUUUUG-CGGUac -5' |
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| 11996 | 3' | -50.5 | NC_003278.1 | + | 15084 | 0.68 | 0.726275 |
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Target: 5'- aCCgUGAcuuUCGCGUAGGucccggcgguGACGCCGg- -3' miRNA: 3'- aGGgACU---AGCGCAUCUu---------UUGCGGUac -5' |
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| 11996 | 3' | -50.5 | NC_003278.1 | + | 14618 | 0.73 | 0.47335 |
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Target: 5'- gUCCUgGGUgGCGUAGAcGAUGCCGUc -3' miRNA: 3'- -AGGGaCUAgCGCAUCUuUUGCGGUAc -5' |
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| 11996 | 3' | -50.5 | NC_003278.1 | + | 19058 | 1.1 | 0.001428 |
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Target: 5'- cUCCCUGAUCGCGUAGAAAACGCCAUGg -3' miRNA: 3'- -AGGGACUAGCGCAUCUUUUGCGGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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