Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 120 | 3' | -59.1 | AC_000006.1 | + | 10957 | 0.66 | 0.395616 |
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Target: 5'- gCGCUGGCGGuCCaGGCcCUCGcGCa -3' miRNA: 3'- gGCGGCCGCUuGGcUCGuGAGCaCGa -5' |
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| 120 | 3' | -59.1 | AC_000006.1 | + | 11523 | 0.67 | 0.377697 |
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Target: 5'- -aGCCGGCGGcgcgaGCUGAGCGaccgCGaGCUg -3' miRNA: 3'- ggCGGCCGCU-----UGGCUCGUga--GCaCGA- -5' |
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| 120 | 3' | -59.1 | AC_000006.1 | + | 22627 | 0.67 | 0.360331 |
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Target: 5'- gCGgCGGCGAG--GGGCuccuCUCGUGCUc -3' miRNA: 3'- gGCgGCCGCUUggCUCGu---GAGCACGA- -5' |
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| 120 | 3' | -59.1 | AC_000006.1 | + | 15455 | 0.67 | 0.343529 |
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Target: 5'- gCCGCCGggccaugauGCGAGCCGcGCGC-CGcGCc -3' miRNA: 3'- -GGCGGC---------CGCUUGGCuCGUGaGCaCGa -5' |
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| 120 | 3' | -59.1 | AC_000006.1 | + | 15934 | 0.69 | 0.282059 |
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Target: 5'- aCCGCCGuGcCGGGCCGcaaCACgCGUGCa -3' miRNA: 3'- -GGCGGC-C-GCUUGGCuc-GUGaGCACGa -5' |
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| 120 | 3' | -59.1 | AC_000006.1 | + | 11634 | 0.69 | 0.275019 |
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Target: 5'- gCCGCCGGCGGcgcccccuACC-AGCGCcCG-GCg -3' miRNA: 3'- -GGCGGCCGCU--------UGGcUCGUGaGCaCGa -5' |
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| 120 | 3' | -59.1 | AC_000006.1 | + | 9769 | 0.76 | 0.087578 |
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Target: 5'- cUCGCCGaGCGcgucgucgccGACCuGGCGCUCGUGCa -3' miRNA: 3'- -GGCGGC-CGC----------UUGGcUCGUGAGCACGa -5' |
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| 120 | 3' | -59.1 | AC_000006.1 | + | 20263 | 0.8 | 0.043745 |
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Target: 5'- -aGCCGGUGAAgcCCGAGUugUUGUGCUg -3' miRNA: 3'- ggCGGCCGCUU--GGCUCGugAGCACGA- -5' |
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| 120 | 3' | -59.1 | AC_000006.1 | + | 7842 | 1.09 | 0.000239 |
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Target: 5'- cCCGCCGGCGAACCGAGCACUCGUGCUg -3' miRNA: 3'- -GGCGGCCGCUUGGCUCGUGAGCACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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