Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 120 | 5' | -56.6 | AC_000006.1 | + | 21546 | 0.66 | 0.429713 |
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Target: 5'- --cGUGUaCCGCA--ACUCGCGcGCGCa -3' miRNA: 3'- uuuCGCA-GGCGUcaUGAGCGU-CGCGa -5' |
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| 120 | 5' | -56.6 | AC_000006.1 | + | 7878 | 1.06 | 0.000548 |
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Target: 5'- aAAAGCGUCCGCAGUACUCGCAGCGCUg -3' miRNA: 3'- -UUUCGCAGGCGUCAUGAGCGUCGCGA- -5' |
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| 120 | 5' | -56.6 | AC_000006.1 | + | 5303 | 0.72 | 0.176572 |
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Target: 5'- aAGAGCGUCCGCAGgcgggACagaggagggaUUGCAGgGCg -3' miRNA: 3'- -UUUCGCAGGCGUCa----UG----------AGCGUCgCGa -5' |
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| 120 | 5' | -56.6 | AC_000006.1 | + | 16910 | 0.68 | 0.345602 |
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Target: 5'- --uGCG-CUGCAG-GCgUCGCAGCGCc -3' miRNA: 3'- uuuCGCaGGCGUCaUG-AGCGUCGCGa -5' |
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| 120 | 5' | -56.6 | AC_000006.1 | + | 5379 | 0.67 | 0.419782 |
|
Target: 5'- aAAAGCGUCCGCuccgcAGUGggCGCAGaCGg- -3' miRNA: 3'- -UUUCGCAGGCG-----UCAUgaGCGUC-GCga -5' |
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| 120 | 5' | -56.6 | AC_000006.1 | + | 15160 | 0.67 | 0.419782 |
|
Target: 5'- --cGCGUCCGCGGccACUucCGCgcuccguggGGCGCUu -3' miRNA: 3'- uuuCGCAGGCGUCa-UGA--GCG---------UCGCGA- -5' |
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| 120 | 5' | -56.6 | AC_000006.1 | + | 10089 | 0.66 | 0.46031 |
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Target: 5'- gGAGGCG--CGCGGgaACUCGCGGaCGCg -3' miRNA: 3'- -UUUCGCagGCGUCa-UGAGCGUC-GCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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