miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1201 5' -44.5 NC_001132.2 + 140016 0.66 0.999998
Target:  5'- gUUUAACGuacACGUcGCGuACGACGCAc -3'
miRNA:   3'- aAAGUUGC---UGUAaUGCuUGUUGCGUu -5'
1201 5' -44.5 NC_001132.2 + 113923 0.66 0.999998
Target:  5'- -gUCAACGACuugcUUACGAugucCGugGUAAa -3'
miRNA:   3'- aaAGUUGCUGu---AAUGCUu---GUugCGUU- -5'
1201 5' -44.5 NC_001132.2 + 115193 0.66 0.999996
Target:  5'- aUUUAGCGACGga--GAACAGCGgGu -3'
miRNA:   3'- aAAGUUGCUGUaaugCUUGUUGCgUu -5'
1201 5' -44.5 NC_001132.2 + 50521 0.67 0.999985
Target:  5'- gUUUUAGCGcCAggGCGuguACGACGCGu -3'
miRNA:   3'- -AAAGUUGCuGUaaUGCu--UGUUGCGUu -5'
1201 5' -44.5 NC_001132.2 + 28705 0.67 0.999979
Target:  5'- --aCGACGACAUccguuaccUACGAaacagauuuuACAACGUAGa -3'
miRNA:   3'- aaaGUUGCUGUA--------AUGCU----------UGUUGCGUU- -5'
1201 5' -44.5 NC_001132.2 + 85334 0.67 0.999979
Target:  5'- cUUCGACGACGUgGCGGgauccuccGCGuuCGCGAg -3'
miRNA:   3'- aAAGUUGCUGUAaUGCU--------UGUu-GCGUU- -5'
1201 5' -44.5 NC_001132.2 + 98304 0.67 0.999979
Target:  5'- --cCAACGACGagacgGCGGACGACGa-- -3'
miRNA:   3'- aaaGUUGCUGUaa---UGCUUGUUGCguu -5'
1201 5' -44.5 NC_001132.2 + 109740 0.67 0.999971
Target:  5'- --cCGAUGACGUUAgGuACGACGUAc -3'
miRNA:   3'- aaaGUUGCUGUAAUgCuUGUUGCGUu -5'
1201 5' -44.5 NC_001132.2 + 8448 0.68 0.999945
Target:  5'- --cCGGcCGAUAUUGCGA-CAACGUAGa -3'
miRNA:   3'- aaaGUU-GCUGUAAUGCUuGUUGCGUU- -5'
1201 5' -44.5 NC_001132.2 + 53799 0.68 0.999902
Target:  5'- uUUUCAACGAaauuGUUaACGAACcGCGCGc -3'
miRNA:   3'- -AAAGUUGCUg---UAA-UGCUUGuUGCGUu -5'
1201 5' -44.5 NC_001132.2 + 27505 0.69 0.999715
Target:  5'- gUUgGACGAUAUaggaUACGAGaCGACGCGu -3'
miRNA:   3'- aAAgUUGCUGUA----AUGCUU-GUUGCGUu -5'
1201 5' -44.5 NC_001132.2 + 153051 0.75 0.977401
Target:  5'- ---aGACGAUA--GCGAGCGACGCGAg -3'
miRNA:   3'- aaagUUGCUGUaaUGCUUGUUGCGUU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.