Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12011 | 5' | -56.4 | NC_003278.1 | + | 32552 | 0.66 | 0.494851 |
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Target: 5'- aCCCGcGcGCgccGGCaaACGCCCUGCAAu -3' miRNA: 3'- aGGGC-CuUGa--CUGgaUGUGGGACGUU- -5' |
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| 12011 | 5' | -56.4 | NC_003278.1 | + | 22824 | 0.67 | 0.422695 |
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Target: 5'- -gCCGGcGCUGgcggGCCUGCcgAUCCUGCAGc -3' miRNA: 3'- agGGCCuUGAC----UGGAUG--UGGGACGUU- -5' |
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| 12011 | 5' | -56.4 | NC_003278.1 | + | 28754 | 0.68 | 0.384403 |
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Target: 5'- -aCUGGAAC-GACCUGCACCa-GCGc -3' miRNA: 3'- agGGCCUUGaCUGGAUGUGGgaCGUu -5' |
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| 12011 | 5' | -56.4 | NC_003278.1 | + | 31757 | 0.69 | 0.323151 |
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Target: 5'- gUCCCGGGcacccuGCUgGACCUGuuCACCC-GCGAc -3' miRNA: 3'- -AGGGCCU------UGA-CUGGAU--GUGGGaCGUU- -5' |
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| 12011 | 5' | -56.4 | NC_003278.1 | + | 12014 | 0.72 | 0.205561 |
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Target: 5'- aUCCCGcccAACggcGACCgcgACGCCCUGCAu -3' miRNA: 3'- -AGGGCc--UUGa--CUGGa--UGUGGGACGUu -5' |
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| 12011 | 5' | -56.4 | NC_003278.1 | + | 23268 | 1.07 | 0.00052 |
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Target: 5'- aUCCCGGAACUGACCUACACCCUGCAAg -3' miRNA: 3'- -AGGGCCUUGACUGGAUGUGGGACGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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