Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12014 | 5' | -51.6 | NC_003278.1 | + | 8278 | 0.66 | 0.761515 |
Target: 5'- cGCCUUGAGGCugAGGggaaugaccaccagGGCGCa -3' miRNA: 3'- -CGGAACUUCG--UCCaaagcaa-------CCGCGa -5' |
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12014 | 5' | -51.6 | NC_003278.1 | + | 27376 | 0.67 | 0.693765 |
Target: 5'- cGgCUUGcuGCAuGGUgcugcgguagUCGUUGGUGCUg -3' miRNA: 3'- -CgGAACuuCGU-CCAa---------AGCAACCGCGA- -5' |
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12014 | 5' | -51.6 | NC_003278.1 | + | 5302 | 0.68 | 0.682155 |
Target: 5'- cGUCgaacUGuuuGCGGGUUUCGUugcgcaUGGUGCUu -3' miRNA: 3'- -CGGa---ACuu-CGUCCAAAGCA------ACCGCGA- -5' |
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12014 | 5' | -51.6 | NC_003278.1 | + | 31401 | 0.69 | 0.570934 |
Target: 5'- cGCCUUGAGGCugauucgcuugcauGGUgucucuccuuuggggUgGUUGGCGCc -3' miRNA: 3'- -CGGAACUUCGu-------------CCAa--------------AgCAACCGCGa -5' |
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12014 | 5' | -51.6 | NC_003278.1 | + | 19370 | 0.69 | 0.565142 |
Target: 5'- cGCCcUGGAGCAGGUcagcaccgCGcUGGcCGCg -3' miRNA: 3'- -CGGaACUUCGUCCAaa------GCaACC-GCGa -5' |
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12014 | 5' | -51.6 | NC_003278.1 | + | 14504 | 0.74 | 0.320785 |
Target: 5'- gGCCUUGAAGCuGGgcgggcaGUUGGCGa- -3' miRNA: 3'- -CGGAACUUCGuCCaaag---CAACCGCga -5' |
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12014 | 5' | -51.6 | NC_003278.1 | + | 25322 | 1.11 | 0.000922 |
Target: 5'- gGCCUUGAAGCAGGUUUCGUUGGCGCUg -3' miRNA: 3'- -CGGAACUUCGUCCAAAGCAACCGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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