Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12017 | 5' | -58.6 | NC_003278.1 | + | 31453 | 0.66 | 0.380852 |
Target: 5'- aUCGCUGGGCgAGugcgagccGCCGAcgUGGUUg -3' miRNA: 3'- aAGCGACCUGgUCu-------CGGCUagACCGA- -5' |
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12017 | 5' | -58.6 | NC_003278.1 | + | 2640 | 0.66 | 0.371792 |
Target: 5'- -gCGcCUGGGCCAG-GUCGGUCcagccGGCg -3' miRNA: 3'- aaGC-GACCUGGUCuCGGCUAGa----CCGa -5' |
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12017 | 5' | -58.6 | NC_003278.1 | + | 9477 | 0.66 | 0.36288 |
Target: 5'- -cCGcCUGGcCCAG-GCCGAgUUGGCg -3' miRNA: 3'- aaGC-GACCuGGUCuCGGCUaGACCGa -5' |
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12017 | 5' | -58.6 | NC_003278.1 | + | 33424 | 0.67 | 0.337044 |
Target: 5'- cUCGCUGGcaACCcuGGCCGucCUGGCc -3' miRNA: 3'- aAGCGACC--UGGucUCGGCuaGACCGa -5' |
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12017 | 5' | -58.6 | NC_003278.1 | + | 8713 | 0.68 | 0.274118 |
Target: 5'- gUUCGCaUGGACUGGucGCCGAUggcaucgCUGGCc -3' miRNA: 3'- -AAGCG-ACCUGGUCu-CGGCUA-------GACCGa -5' |
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12017 | 5' | -58.6 | NC_003278.1 | + | 28498 | 0.69 | 0.228242 |
Target: 5'- -aCGCUGGgugggcaccACCAGAcGCCGcgCgGGCUg -3' miRNA: 3'- aaGCGACC---------UGGUCU-CGGCuaGaCCGA- -5' |
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12017 | 5' | -58.6 | NC_003278.1 | + | 31176 | 0.7 | 0.204736 |
Target: 5'- -cCGCUGGGCCAGGGCgGc-CUGGa- -3' miRNA: 3'- aaGCGACCUGGUCUCGgCuaGACCga -5' |
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12017 | 5' | -58.6 | NC_003278.1 | + | 27490 | 1.05 | 0.00038 |
Target: 5'- cUUCGCUGGACCAGAGCCGAUCUGGCUg -3' miRNA: 3'- -AAGCGACCUGGUCUCGGCUAGACCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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