miRNA: 3'- ucuuGCUaaCCGCaUgCUCCUGGACa -5'
miRNA: 3'- -UCUUGCUaaccGCAugCUCCUGGaca -5'
miRNA: 3'- -UCUUGCUAaccGCAugCUCCUGGACa -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1202 | 3' | -52.2 | NC_001132.2 | + | 33182 | 0.68 | 0.954929 |
|
Target: 5'- ---uCGAggGGCGcGgGAGGACCUGc -3' miRNA: 3'- ucuuGCUaaCCGCaUgCUCCUGGACa -5' |
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| 1202 | 3' | -52.2 | NC_001132.2 | + | 51611 | 0.66 | 0.987198 |
|
Target: 5'- aAGAACGAaa--CGUugGGGGACCa-- -3' miRNA: 3'- -UCUUGCUaaccGCAugCUCCUGGaca -5' |
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| 1202 | 3' | -52.2 | NC_001132.2 | + | 87754 | 0.76 | 0.640827 |
|
Target: 5'- uAGAACGAU---UGUugGAGGACCUGg -3' miRNA: 3'- -UCUUGCUAaccGCAugCUCCUGGACa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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