Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12023 | 3' | -64.2 | NC_003278.1 | + | 19915 | 0.74 | 0.052328 |
Target: 5'- uGCGCuCGAUGCGagucaCCGccuGGGUGGCCGAc -3' miRNA: 3'- -UGCG-GCUGCGCg----GGC---UCCACCGGCUc -5' |
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12023 | 3' | -64.2 | NC_003278.1 | + | 32671 | 0.76 | 0.035197 |
Target: 5'- gACGCCGAUgaugGCGCCCGAGGUG-UCGGu -3' miRNA: 3'- -UGCGGCUG----CGCGGGCUCCACcGGCUc -5' |
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12023 | 3' | -64.2 | NC_003278.1 | + | 30350 | 1.07 | 0.00013 |
Target: 5'- aACGCCGACGCGCCCGAGGUGGCCGAGu -3' miRNA: 3'- -UGCGGCUGCGCGGGCUCCACCGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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