Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12023 | 5' | -48.3 | NC_003278.1 | + | 20642 | 0.66 | 0.929135 |
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Target: 5'- gGUCgcgGCGACccucgcgGCCUCGc---CCUCGGg -3' miRNA: 3'- -CAGa--UGCUGa------UGGAGCuuuuGGAGUC- -5' |
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| 12023 | 5' | -48.3 | NC_003278.1 | + | 1124 | 0.67 | 0.916331 |
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Target: 5'- ---aGCGGCccUAUC-CGGAAACCUCGGa -3' miRNA: 3'- cagaUGCUG--AUGGaGCUUUUGGAGUC- -5' |
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| 12023 | 5' | -48.3 | NC_003278.1 | + | 5047 | 0.67 | 0.902238 |
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Target: 5'- cUCgGCGAUcGCUUCGAcgGCCUCGc -3' miRNA: 3'- cAGaUGCUGaUGGAGCUuuUGGAGUc -5' |
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| 12023 | 5' | -48.3 | NC_003278.1 | + | 26385 | 0.68 | 0.851653 |
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Target: 5'- uGUUUGCgGGCUGCCUCGcgGGCUugcuccgUCGGg -3' miRNA: 3'- -CAGAUG-CUGAUGGAGCuuUUGG-------AGUC- -5' |
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| 12023 | 5' | -48.3 | NC_003278.1 | + | 2153 | 0.7 | 0.782576 |
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Target: 5'- -gCU-CGGCaUACCUCGAGcAGCCUCGu -3' miRNA: 3'- caGAuGCUG-AUGGAGCUU-UUGGAGUc -5' |
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| 12023 | 5' | -48.3 | NC_003278.1 | + | 15208 | 0.7 | 0.760729 |
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Target: 5'- cUCgUACGAuCUACCUCGGuggGGACgUCAGc -3' miRNA: 3'- cAG-AUGCU-GAUGGAGCU---UUUGgAGUC- -5' |
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| 12023 | 5' | -48.3 | NC_003278.1 | + | 10388 | 0.71 | 0.726836 |
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Target: 5'- aUCUGCaucGGCccaucgACCUCGAugGCCUCGGu -3' miRNA: 3'- cAGAUG---CUGa-----UGGAGCUuuUGGAGUC- -5' |
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| 12023 | 5' | -48.3 | NC_003278.1 | + | 10466 | 0.71 | 0.703663 |
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Target: 5'- aUCUGCGGCgu-CUCGAGcaugacGGCCUCGGu -3' miRNA: 3'- cAGAUGCUGaugGAGCUU------UUGGAGUC- -5' |
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| 12023 | 5' | -48.3 | NC_003278.1 | + | 18021 | 0.71 | 0.696641 |
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Target: 5'- -cCUACGACUACaccccggugccgccgCUCGAGGACCUg-- -3' miRNA: 3'- caGAUGCUGAUG---------------GAGCUUUUGGAguc -5' |
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| 12023 | 5' | -48.3 | NC_003278.1 | + | 30385 | 1.09 | 0.003446 |
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Target: 5'- gGUCUACGACUACCUCGAAAACCUCAGc -3' miRNA: 3'- -CAGAUGCUGAUGGAGCUUUUGGAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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