miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12025 5' -56.5 NC_003278.1 + 27670 0.66 0.553795
Target:  5'- uGGGGGC-GCuGuuuGAGCAGGUGCgCGg -3'
miRNA:   3'- gCUCCUGaCGcCu--CUCGUCUAUGgGC- -5'
12025 5' -56.5 NC_003278.1 + 22833 0.66 0.499789
Target:  5'- cCGAGGGCgaUGuCGGuGAGCAcGUcGCCCa -3'
miRNA:   3'- -GCUCCUG--AC-GCCuCUCGUcUA-UGGGc -5'
12025 5' -56.5 NC_003278.1 + 10539 0.67 0.458324
Target:  5'- uCGAGGGC-GCGGAGAauCGGcccUGCCCa -3'
miRNA:   3'- -GCUCCUGaCGCCUCUc-GUCu--AUGGGc -5'
12025 5' -56.5 NC_003278.1 + 4854 0.68 0.415885
Target:  5'- gCGAGGgucccguucuuggcGCUGaaGGAGAGCGccucGGUACCCa -3'
miRNA:   3'- -GCUCC--------------UGACg-CCUCUCGU----CUAUGGGc -5'
12025 5' -56.5 NC_003278.1 + 24813 0.69 0.363504
Target:  5'- -aAGGGCUGUGGAG-GUAGAUAaagaaCCa -3'
miRNA:   3'- gcUCCUGACGCCUCuCGUCUAUg----GGc -5'
12025 5' -56.5 NC_003278.1 + 31176 1.09 0.000428
Target:  5'- gCGAGGACUGCGGAGAGCAGAUACCCGa -3'
miRNA:   3'- -GCUCCUGACGCCUCUCGUCUAUGGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.