Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12025 | 5' | -56.5 | NC_003278.1 | + | 27670 | 0.66 | 0.553795 |
Target: 5'- uGGGGGC-GCuGuuuGAGCAGGUGCgCGg -3' miRNA: 3'- gCUCCUGaCGcCu--CUCGUCUAUGgGC- -5' |
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12025 | 5' | -56.5 | NC_003278.1 | + | 22833 | 0.66 | 0.499789 |
Target: 5'- cCGAGGGCgaUGuCGGuGAGCAcGUcGCCCa -3' miRNA: 3'- -GCUCCUG--AC-GCCuCUCGUcUA-UGGGc -5' |
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12025 | 5' | -56.5 | NC_003278.1 | + | 10539 | 0.67 | 0.458324 |
Target: 5'- uCGAGGGC-GCGGAGAauCGGcccUGCCCa -3' miRNA: 3'- -GCUCCUGaCGCCUCUc-GUCu--AUGGGc -5' |
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12025 | 5' | -56.5 | NC_003278.1 | + | 4854 | 0.68 | 0.415885 |
Target: 5'- gCGAGGgucccguucuuggcGCUGaaGGAGAGCGccucGGUACCCa -3' miRNA: 3'- -GCUCC--------------UGACg-CCUCUCGU----CUAUGGGc -5' |
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12025 | 5' | -56.5 | NC_003278.1 | + | 24813 | 0.69 | 0.363504 |
Target: 5'- -aAGGGCUGUGGAG-GUAGAUAaagaaCCa -3' miRNA: 3'- gcUCCUGACGCCUCuCGUCUAUg----GGc -5' |
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12025 | 5' | -56.5 | NC_003278.1 | + | 31176 | 1.09 | 0.000428 |
Target: 5'- gCGAGGACUGCGGAGAGCAGAUACCCGa -3' miRNA: 3'- -GCUCCUGACGCCUCUCGUCUAUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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