Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12026 | 3' | -52.2 | NC_003278.1 | + | 10252 | 0.66 | 0.713387 |
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Target: 5'- uGCugCGguCGAGCauGUCGGCCUCGAa -3' miRNA: 3'- -UGugGUacGUUCGcuUAGUCGGAGUU- -5' |
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| 12026 | 3' | -52.2 | NC_003278.1 | + | 33080 | 0.66 | 0.713387 |
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Target: 5'- uCGCCuucgGCAugcGCGGAUCGGCCa--- -3' miRNA: 3'- uGUGGua--CGUu--CGCUUAGUCGGaguu -5' |
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| 12026 | 3' | -52.2 | NC_003278.1 | + | 19940 | 0.66 | 0.713387 |
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Target: 5'- aGCGCC-UGCAucuGCGGAUCGuccuuGCgCUCGAu -3' miRNA: 3'- -UGUGGuACGUu--CGCUUAGU-----CG-GAGUU- -5' |
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| 12026 | 3' | -52.2 | NC_003278.1 | + | 19257 | 0.66 | 0.689991 |
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Target: 5'- cGCAUCAUGCAaggcAGCGAcgCgacggcccgGGCgCUCAAg -3' miRNA: 3'- -UGUGGUACGU----UCGCUuaG---------UCG-GAGUU- -5' |
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| 12026 | 3' | -52.2 | NC_003278.1 | + | 1762 | 0.67 | 0.642524 |
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Target: 5'- aGCGCCGUGCGcAGCGcGUCGaugucuuCCUCAu -3' miRNA: 3'- -UGUGGUACGU-UCGCuUAGUc------GGAGUu -5' |
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| 12026 | 3' | -52.2 | NC_003278.1 | + | 17013 | 0.67 | 0.642524 |
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Target: 5'- cGCACCAUcGCcaccGCGAucAUCGGCCUgGu -3' miRNA: 3'- -UGUGGUA-CGuu--CGCU--UAGUCGGAgUu -5' |
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| 12026 | 3' | -52.2 | NC_003278.1 | + | 14342 | 0.68 | 0.583024 |
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Target: 5'- aGCGCgGUaGCGcuGGCGAAUCAGCUUgGu -3' miRNA: 3'- -UGUGgUA-CGU--UCGCUUAGUCGGAgUu -5' |
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| 12026 | 3' | -52.2 | NC_003278.1 | + | 10434 | 0.68 | 0.571222 |
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Target: 5'- gGCGCCcgGCcaauGGCuGA-CAGCCUCGAg -3' miRNA: 3'- -UGUGGuaCGu---UCGcUUaGUCGGAGUU- -5' |
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| 12026 | 3' | -52.2 | NC_003278.1 | + | 19033 | 0.73 | 0.28156 |
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Target: 5'- uGCugCAgaaGCGGGCGAAggCAGCCUCc- -3' miRNA: 3'- -UGugGUa--CGUUCGCUUa-GUCGGAGuu -5' |
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| 12026 | 3' | -52.2 | NC_003278.1 | + | 31336 | 1.06 | 0.00125 |
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Target: 5'- gACACCAUGCAAGCGAAUCAGCCUCAAg -3' miRNA: 3'- -UGUGGUACGUUCGCUUAGUCGGAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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