Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12029 | 3' | -54.9 | NC_003278.1 | + | 23246 | 0.68 | 0.502095 |
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Target: 5'- gGCGGuu-CCACUCgGCG-CggGCGGCGc -3' miRNA: 3'- -CGCCuuuGGUGAG-UGCuGa-CGCCGCu -5' |
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| 12029 | 3' | -54.9 | NC_003278.1 | + | 26151 | 0.69 | 0.420319 |
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Target: 5'- aGCGGAcuauuGCCGgUUACGAaggugGUGGCGGg -3' miRNA: 3'- -CGCCUu----UGGUgAGUGCUga---CGCCGCU- -5' |
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| 12029 | 3' | -54.9 | NC_003278.1 | + | 27098 | 0.73 | 0.26215 |
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Target: 5'- aGCGGAu-CCGCaUC-CGGCUGUGGCa- -3' miRNA: 3'- -CGCCUuuGGUG-AGuGCUGACGCCGcu -5' |
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| 12029 | 3' | -54.9 | NC_003278.1 | + | 29692 | 0.66 | 0.60135 |
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Target: 5'- uGUGGAAGCUGCU--CGGCcggGCGGaCGAg -3' miRNA: 3'- -CGCCUUUGGUGAguGCUGa--CGCC-GCU- -5' |
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| 12029 | 3' | -54.9 | NC_003278.1 | + | 32130 | 1.11 | 0.000492 |
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Target: 5'- gGCGGAAACCACUCACGACUGCGGCGAg -3' miRNA: 3'- -CGCCUUUGGUGAGUGCUGACGCCGCU- -5' |
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| 12029 | 3' | -54.9 | NC_003278.1 | + | 33203 | 0.67 | 0.534542 |
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Target: 5'- uGCGGuu-CCGC---CGGCUGCGGcCGAg -3' miRNA: 3'- -CGCCuuuGGUGaguGCUGACGCC-GCU- -5' |
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| 12029 | 3' | -54.9 | NC_003278.1 | + | 33896 | 0.72 | 0.298504 |
|
Target: 5'- cGCGGAAGCCGCUgGCGGCaaucugGuuGCGu -3' miRNA: 3'- -CGCCUUUGGUGAgUGCUGa-----CgcCGCu -5' |
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| 12029 | 3' | -54.9 | NC_003278.1 | + | 35503 | 0.7 | 0.38256 |
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Target: 5'- gGCGGgcGCUGCUgGUGGCuuucUGCGGCGAu -3' miRNA: 3'- -CGCCuuUGGUGAgUGCUG----ACGCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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