Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12029 | 5' | -57.9 | NC_003278.1 | + | 26674 | 0.7 | 0.247199 |
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Target: 5'- --aGGCCCAGgcggGCGGcGGCGCauucgcgUCCg -3' miRNA: 3'- agaCCGGGUCag--CGCCuUCGCGa------AGG- -5' |
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| 12029 | 5' | -57.9 | NC_003278.1 | + | 3993 | 0.7 | 0.234508 |
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Target: 5'- cUUGaGCgCCAGgCGCGGAuccAGCGCUaCCa -3' miRNA: 3'- aGAC-CG-GGUCaGCGCCU---UCGCGAaGG- -5' |
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| 12029 | 5' | -57.9 | NC_003278.1 | + | 12922 | 0.71 | 0.216502 |
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Target: 5'- gCUGGCgCCAGagcauuUCGCGGuauGCGUUUUCc -3' miRNA: 3'- aGACCG-GGUC------AGCGCCuu-CGCGAAGG- -5' |
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| 12029 | 5' | -57.9 | NC_003278.1 | + | 19720 | 0.72 | 0.199693 |
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Target: 5'- gCUGGCUgaGGUUGCGuAAGCGCUgcgCCu -3' miRNA: 3'- aGACCGGg-UCAGCGCcUUCGCGAa--GG- -5' |
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| 12029 | 5' | -57.9 | NC_003278.1 | + | 30132 | 0.72 | 0.184032 |
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Target: 5'- --aGGCCCGcgUGCuGGAGUGCUUCCg -3' miRNA: 3'- agaCCGGGUcaGCGcCUUCGCGAAGG- -5' |
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| 12029 | 5' | -57.9 | NC_003278.1 | + | 19527 | 0.72 | 0.174203 |
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Target: 5'- -aUGGCCCgguuguAGUCGCGGGugAGCGCg--- -3' miRNA: 3'- agACCGGG------UCAGCGCCU--UCGCGaagg -5' |
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| 12029 | 5' | -57.9 | NC_003278.1 | + | 32165 | 1.12 | 0.000195 |
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Target: 5'- gUCUGGCCCAGUCGCGGAAGCGCUUCCu -3' miRNA: 3'- -AGACCGGGUCAGCGCCUUCGCGAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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