Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1203 | 3' | -48.6 | NC_001132.2 | + | 11171 | 0.66 | 0.999552 |
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Target: 5'- cGUUCGuAUGA-AGACGAGCUGgagACAc -3' miRNA: 3'- -CAAGC-UAUUgUCUGCUCGGCacaUGU- -5' |
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| 1203 | 3' | -48.6 | NC_001132.2 | + | 33430 | 0.66 | 0.999552 |
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Target: 5'- cUUCGuacGGCAGaACGGGacCCGUGUAUAa -3' miRNA: 3'- cAAGCua-UUGUC-UGCUC--GGCACAUGU- -5' |
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| 1203 | 3' | -48.6 | NC_001132.2 | + | 104919 | 0.66 | 0.999145 |
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Target: 5'- --aCGAUGAUuaAGACGucGGCCGUcuuuGUACGa -3' miRNA: 3'- caaGCUAUUG--UCUGC--UCGGCA----CAUGU- -5' |
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| 1203 | 3' | -48.6 | NC_001132.2 | + | 6131 | 0.66 | 0.998953 |
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Target: 5'- cGUUCGGggaacgGACGGAuaCGGGCCc-GUACAa -3' miRNA: 3'- -CAAGCUa-----UUGUCU--GCUCGGcaCAUGU- -5' |
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| 1203 | 3' | -48.6 | NC_001132.2 | + | 129185 | 0.67 | 0.998674 |
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Target: 5'- --aCGAUAAUAGuucuaaaauGCCGUGUACGa -3' miRNA: 3'- caaGCUAUUGUCugcu-----CGGCACAUGU- -5' |
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| 1203 | 3' | -48.6 | NC_001132.2 | + | 13122 | 0.67 | 0.997771 |
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Target: 5'- --aCGGUuacgcuggacgcGACGGACGAuGCCGUucGUACGg -3' miRNA: 3'- caaGCUA------------UUGUCUGCU-CGGCA--CAUGU- -5' |
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| 1203 | 3' | -48.6 | NC_001132.2 | + | 137880 | 0.67 | 0.997771 |
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Target: 5'- --cCGAacACAaACGGGCCGUGUAa- -3' miRNA: 3'- caaGCUauUGUcUGCUCGGCACAUgu -5' |
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| 1203 | 3' | -48.6 | NC_001132.2 | + | 4517 | 0.71 | 0.975485 |
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Target: 5'- uUUCuacUAACGGACGuccgagaAGCCGUGUACGc -3' miRNA: 3'- cAAGcu-AUUGUCUGC-------UCGGCACAUGU- -5' |
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| 1203 | 3' | -48.6 | NC_001132.2 | + | 34641 | 0.71 | 0.966884 |
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Target: 5'- uGUUCGAUAauuuuaaaAUAGAguuCGaAGCCGUGUAUAa -3' miRNA: 3'- -CAAGCUAU--------UGUCU---GC-UCGGCACAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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