Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12031 | 5' | -58.9 | NC_003278.1 | + | 29896 | 0.66 | 0.36288 |
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Target: 5'- -cCCAGUCGaaGGAcuuggcGGCGCCgccGGCGc -3' miRNA: 3'- uaGGUCAGCgaCCUa-----CCGUGGa--CCGU- -5' |
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| 12031 | 5' | -58.9 | NC_003278.1 | + | 9692 | 0.66 | 0.344652 |
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Target: 5'- gGUCCucgaucaAGcguUCGC-GGAUGGCcagcGCCUGGCGc -3' miRNA: 3'- -UAGG-------UC---AGCGaCCUACCG----UGGACCGU- -5' |
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| 12031 | 5' | -58.9 | NC_003278.1 | + | 16805 | 0.66 | 0.327913 |
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Target: 5'- -gCCAGUCGCgcaguUGGcgGcgguacgccugcaGCGCCUGGUAc -3' miRNA: 3'- uaGGUCAGCG-----ACCuaC-------------CGUGGACCGU- -5' |
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| 12031 | 5' | -58.9 | NC_003278.1 | + | 8706 | 0.67 | 0.28208 |
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Target: 5'- uUCCAGcguUCGCaUGGAcUGGuCGCCgaUGGCAu -3' miRNA: 3'- uAGGUC---AGCG-ACCU-ACC-GUGG--ACCGU- -5' |
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| 12031 | 5' | -58.9 | NC_003278.1 | + | 13731 | 0.69 | 0.228242 |
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Target: 5'- cUCCGucgaUCGCUGGGacaGCACCUGGCc -3' miRNA: 3'- uAGGUc---AGCGACCUac-CGUGGACCGu -5' |
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| 12031 | 5' | -58.9 | NC_003278.1 | + | 3926 | 0.7 | 0.183356 |
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Target: 5'- -cCUGGUagCGCUGGAUccGCGCCUGGCGc -3' miRNA: 3'- uaGGUCA--GCGACCUAc-CGUGGACCGU- -5' |
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| 12031 | 5' | -58.9 | NC_003278.1 | + | 19730 | 0.72 | 0.134451 |
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Target: 5'- cAUCCAGagcgcugccgCGCUGGccGGCACC-GGCAu -3' miRNA: 3'- -UAGGUCa---------GCGACCuaCCGUGGaCCGU- -5' |
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| 12031 | 5' | -58.9 | NC_003278.1 | + | 33065 | 1.06 | 0.000316 |
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Target: 5'- cAUCCAGUCGCUGGAUGGCACCUGGCAc -3' miRNA: 3'- -UAGGUCAGCGACCUACCGUGGACCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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