miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12039 5' -48.9 NC_003298.1 + 35977 0.66 0.933115
Target:  5'- -cAUCGaaacuaAUcACAGAGUcacgcUGACCGGGGc -3'
miRNA:   3'- caUAGCg-----UGuUGUCUCA-----AUUGGCCCU- -5'
12039 5' -48.9 NC_003298.1 + 2450 0.66 0.914177
Target:  5'- gGUAUCGUucagcgcagACGACA-AGUUAaaGCCGGGu -3'
miRNA:   3'- -CAUAGCG---------UGUUGUcUCAAU--UGGCCCu -5'
12039 5' -48.9 NC_003298.1 + 5497 0.67 0.876308
Target:  5'- ---aCGCACAGCcuuaaAGAGcUUAccagcgucuGCCGGGAu -3'
miRNA:   3'- cauaGCGUGUUG-----UCUC-AAU---------UGGCCCU- -5'
12039 5' -48.9 NC_003298.1 + 8160 1.1 0.002322
Target:  5'- aGUAUCGCACAACAGAGUUAACCGGGAa -3'
miRNA:   3'- -CAUAGCGUGUUGUCUCAAUUGGCCCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.