Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12043 | 3' | -45.2 | NC_003298.1 | + | 14032 | 0.66 | 0.993554 |
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Target: 5'- aGCAAcGu---ACUCGcGGGUAUCCAGUu -3' miRNA: 3'- -CGUUaCuuucUGAGU-CUCAUAGGUCG- -5' |
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| 12043 | 3' | -45.2 | NC_003298.1 | + | 26163 | 0.66 | 0.992403 |
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Target: 5'- uGCAuguuacGUGAAGucaACUCAGAGgAUgCAGCu -3' miRNA: 3'- -CGU------UACUUUc--UGAGUCUCaUAgGUCG- -5' |
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| 12043 | 3' | -45.2 | NC_003298.1 | + | 30447 | 0.67 | 0.984008 |
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Target: 5'- --cAUGAGAGACuUCAcGGUAccgaCCAGCg -3' miRNA: 3'- cguUACUUUCUG-AGUcUCAUa---GGUCG- -5' |
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| 12043 | 3' | -45.2 | NC_003298.1 | + | 12776 | 0.67 | 0.984008 |
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Target: 5'- ----cGAAcuGgUCAGcAGUGUCCGGCa -3' miRNA: 3'- cguuaCUUucUgAGUC-UCAUAGGUCG- -5' |
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| 12043 | 3' | -45.2 | NC_003298.1 | + | 6351 | 0.69 | 0.943503 |
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Target: 5'- uGCGGUGAGGuuCUCAGuGUGUUCAcacGCu -3' miRNA: 3'- -CGUUACUUUcuGAGUCuCAUAGGU---CG- -5' |
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| 12043 | 3' | -45.2 | NC_003298.1 | + | 158 | 0.72 | 0.873032 |
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Target: 5'- aGUGAUGGcugccuauugGAGACUUaaAGAGUGUCCuAGUg -3' miRNA: 3'- -CGUUACU----------UUCUGAG--UCUCAUAGG-UCG- -5' |
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| 12043 | 3' | -45.2 | NC_003298.1 | + | 38097 | 0.72 | 0.873032 |
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Target: 5'- aGUGAUGGcugccuauugGAGACUUaaAGAGUGUCCuAGUg -3' miRNA: 3'- -CGUUACU----------UUCUGAG--UCUCAUAGG-UCG- -5' |
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| 12043 | 3' | -45.2 | NC_003298.1 | + | 26548 | 0.75 | 0.703368 |
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Target: 5'- cGguAUGGAAcGACUCAGGGgaGUCCGGg -3' miRNA: 3'- -CguUACUUU-CUGAGUCUCa-UAGGUCg -5' |
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| 12043 | 3' | -45.2 | NC_003298.1 | + | 17418 | 1.18 | 0.001975 |
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Target: 5'- cGCAAUGAAAGACUCAGAGUAUCCAGCa -3' miRNA: 3'- -CGUUACUUUCUGAGUCUCAUAGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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