miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12046 3' -56.8 NC_003298.1 + 30403 0.66 0.481701
Target:  5'- cUCUGCgGaUGuCAGCAUCGUCAGaCCc -3'
miRNA:   3'- aAGACGgUcAU-GUCGUGGCAGUC-GGc -5'
12046 3' -56.8 NC_003298.1 + 2016 0.68 0.355678
Target:  5'- gUCUGCCAGUAguccaugcguucUAGCGCCGUguuuccugCuGCCu -3'
miRNA:   3'- aAGACGGUCAU------------GUCGUGGCA--------GuCGGc -5'
12046 3' -56.8 NC_003298.1 + 21327 1.05 0.000623
Target:  5'- gUUCUGCCAGUACAGCACCGUCAGCCGc -3'
miRNA:   3'- -AAGACGGUCAUGUCGUGGCAGUCGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.