Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12047 | 3' | -43.6 | NC_003298.1 | + | 5725 | 0.68 | 0.989412 |
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Target: 5'- aGUGUCUuaaAGUgggagaccuuaaguaGUCUCCCgUUAGUGa -3' miRNA: 3'- -CAUAGAuaaUCA---------------CAGAGGGaAAUCAC- -5' |
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| 12047 | 3' | -43.6 | NC_003298.1 | + | 10654 | 0.69 | 0.980159 |
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Target: 5'- -----------aGUCUCCCUUUAGUGa -3' miRNA: 3'- cauagauaaucaCAGAGGGAAAUCAC- -5' |
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| 12047 | 3' | -43.6 | NC_003298.1 | + | 19742 | 0.71 | 0.949809 |
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Target: 5'- --------gAG-GUCUCCCUUUAGUGa -3' miRNA: 3'- cauagauaaUCaCAGAGGGAAAUCAC- -5' |
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| 12047 | 3' | -43.6 | NC_003298.1 | + | 20783 | 0.8 | 0.551355 |
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Target: 5'- -cAUCUA-UGGUcUCUCCCUUUAGUGa -3' miRNA: 3'- caUAGAUaAUCAcAGAGGGAAAUCAC- -5' |
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| 12047 | 3' | -43.6 | NC_003298.1 | + | 10254 | 0.8 | 0.551354 |
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Target: 5'- --cUCUAUUGGUGUCUgCCUUguaGGUGg -3' miRNA: 3'- cauAGAUAAUCACAGAgGGAAa--UCAC- -5' |
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| 12047 | 3' | -43.6 | NC_003298.1 | + | 25505 | 1.11 | 0.007554 |
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Target: 5'- cGUAUCUAUUAGUGUCUCCCUUUAGUGa -3' miRNA: 3'- -CAUAGAUAAUCACAGAGGGAAAUCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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