Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12055 | 5' | -48.9 | NC_003298.1 | + | 21874 | 0.67 | 0.862433 |
Target: 5'- aGCUGCU---GGCGCUAUCGUgcucccaAAGGUg -3' miRNA: 3'- -CGAUGAaggUCGUGAUGGCAa------UUCCA- -5' |
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12055 | 5' | -48.9 | NC_003298.1 | + | 6851 | 0.68 | 0.85256 |
Target: 5'- -gUugUUgCCAGCGCUuaucgaaACCGUUcAGGUg -3' miRNA: 3'- cgAugAA-GGUCGUGA-------UGGCAAuUCCA- -5' |
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12055 | 5' | -48.9 | NC_003298.1 | + | 31685 | 0.7 | 0.749801 |
Target: 5'- aGUUGCUUCCcuuagacgcuGCACUAUaucagcaCGUUGAGGUc -3' miRNA: 3'- -CGAUGAAGGu---------CGUGAUG-------GCAAUUCCA- -5' |
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12055 | 5' | -48.9 | NC_003298.1 | + | 21691 | 1.1 | 0.002457 |
Target: 5'- gGCUACUUCCAGCACUACCGUUAAGGUu -3' miRNA: 3'- -CGAUGAAGGUCGUGAUGGCAAUUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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