Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1207 | 3' | -48.1 | NC_001132.2 | + | 10900 | 0.66 | 0.999676 |
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Target: 5'- -aCAUGAGGaaagacguGUGUcaGGugGUAAUGCc -3' miRNA: 3'- agGUACUCUgu------CACA--UCugCAUUGCG- -5' |
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| 1207 | 3' | -48.1 | NC_001132.2 | + | 9653 | 0.67 | 0.999365 |
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Target: 5'- cCCAcGAGACAGgucuugucGUAcGACGUAAagGCg -3' miRNA: 3'- aGGUaCUCUGUCa-------CAU-CUGCAUUg-CG- -5' |
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| 1207 | 3' | -48.1 | NC_001132.2 | + | 113509 | 0.67 | 0.999323 |
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Target: 5'- gUCgAUGuuuggauauaaggcGGACGGUGUAGGCau-ACGCc -3' miRNA: 3'- -AGgUAC--------------UCUGUCACAUCUGcauUGCG- -5' |
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| 1207 | 3' | -48.1 | NC_001132.2 | + | 140201 | 0.68 | 0.998279 |
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Target: 5'- -aCAUGAGACGGacgaucGGAgGUAGCGUc -3' miRNA: 3'- agGUACUCUGUCaca---UCUgCAUUGCG- -5' |
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| 1207 | 3' | -48.1 | NC_001132.2 | + | 12371 | 0.7 | 0.987226 |
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Target: 5'- gCCGgagaUGGGACAcuaUAGACGUAAUGCg -3' miRNA: 3'- aGGU----ACUCUGUcacAUCUGCAUUGCG- -5' |
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| 1207 | 3' | -48.1 | NC_001132.2 | + | 9380 | 0.72 | 0.968408 |
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Target: 5'- aUCCAUcGAGACGGcGUcccGGACGUcacgGACGUc -3' miRNA: 3'- -AGGUA-CUCUGUCaCA---UCUGCA----UUGCG- -5' |
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| 1207 | 3' | -48.1 | NC_001132.2 | + | 160623 | 0.73 | 0.953822 |
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Target: 5'- cUCCAaggagcgucgcUG-GACAGUcggacGUAGACGUGGCGUc -3' miRNA: 3'- -AGGU-----------ACuCUGUCA-----CAUCUGCAUUGCG- -5' |
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| 1207 | 3' | -48.1 | NC_001132.2 | + | 7185 | 1.15 | 0.00883 |
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Target: 5'- cUCCAUGAGACAGUGUAGACGUAACGCg -3' miRNA: 3'- -AGGUACUCUGUCACAUCUGCAUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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