miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1209 3' -46.2 NC_001132.2 + 7916 0.66 0.999987
Target:  5'- ---cUUCGCAccgUGCUCCGcu-ACAUGUa -3'
miRNA:   3'- gucaAAGCGU---AUGAGGCauuUGUGCA- -5'
1209 3' -46.2 NC_001132.2 + 11335 0.66 0.999966
Target:  5'- gAGUUcuccUCGCGauucaaUAcCUCCGUGcAACGCGUg -3'
miRNA:   3'- gUCAA----AGCGU------AU-GAGGCAU-UUGUGCA- -5'
1209 3' -46.2 NC_001132.2 + 62486 0.68 0.999766
Target:  5'- -cGUUUUGCAUaagGCUCCGUccauGGCACc- -3'
miRNA:   3'- guCAAAGCGUA---UGAGGCAu---UUGUGca -5'
1209 3' -46.2 NC_001132.2 + 111681 0.69 0.999259
Target:  5'- cCAGUUUUGaGUACgCCGUGgacuuGACACGUu -3'
miRNA:   3'- -GUCAAAGCgUAUGaGGCAU-----UUGUGCA- -5'
1209 3' -46.2 NC_001132.2 + 4288 1.08 0.030972
Target:  5'- aCAGUUUCGCAUACUCCGUAAACACGUu -3'
miRNA:   3'- -GUCAAAGCGUAUGAGGCAUUUGUGCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.