miRNA display CGI


Results 1 - 3 of 3 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1209 5' -54.7 NC_001132.2 + 10963 0.76 0.502638
Target:  5'- cGGACGACUUg--GGAGAGaUCGUGCGGg -3'
miRNA:   3'- -CUUGCUGAGaggCCUCUC-AGCACGCU- -5'
1209 5' -54.7 NC_001132.2 + 157704 0.84 0.181676
Target:  5'- -cACGACUCUCCGGAGAGUCGUu--- -3'
miRNA:   3'- cuUGCUGAGAGGCCUCUCAGCAcgcu -5'
1209 5' -54.7 NC_001132.2 + 4254 1.09 0.004603
Target:  5'- gGAACGACUCUCCGGAGAGUCGUGCGAa -3'
miRNA:   3'- -CUUGCUGAGAGGCCUCUCAGCACGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.