Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12096 | 5' | -51.7 | NC_003309.1 | + | 654 | 1.09 | 0.002527 |
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Target: 5'- cCACGAACGACGUCUCGCGCGAACUUGa -3' miRNA: 3'- -GUGCUUGCUGCAGAGCGCGCUUGAAC- -5' |
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| 12096 | 5' | -51.7 | NC_003309.1 | + | 9095 | 0.69 | 0.747327 |
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Target: 5'- gGCGGACGACaucugCUCGCGC-AGCUc- -3' miRNA: 3'- gUGCUUGCUGca---GAGCGCGcUUGAac -5' |
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| 12096 | 5' | -51.7 | NC_003309.1 | + | 18416 | 0.7 | 0.67102 |
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Target: 5'- gACGAGCGcgaccguguagcGCGUCUUGCGCucGCUg- -3' miRNA: 3'- gUGCUUGC------------UGCAGAGCGCGcuUGAac -5' |
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| 12096 | 5' | -51.7 | NC_003309.1 | + | 22444 | 0.67 | 0.836183 |
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Target: 5'- gCACGcugcCGGCGUCgagcgcggCGCGCGAGauCUUGc -3' miRNA: 3'- -GUGCuu--GCUGCAGa-------GCGCGCUU--GAAC- -5' |
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| 12096 | 5' | -51.7 | NC_003309.1 | + | 26963 | 0.7 | 0.725949 |
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Target: 5'- aGgGAGuucCGACGUCagCGCGCGAACa-- -3' miRNA: 3'- gUgCUU---GCUGCAGa-GCGCGCUUGaac -5' |
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| 12096 | 5' | -51.7 | NC_003309.1 | + | 32326 | 0.66 | 0.893373 |
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Target: 5'- gGCGGACGAUGUCaaGCGagaugcCGAGCg-- -3' miRNA: 3'- gUGCUUGCUGCAGagCGC------GCUUGaac -5' |
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| 12096 | 5' | -51.7 | NC_003309.1 | + | 38848 | 0.76 | 0.368739 |
|
Target: 5'- -cCGAACgGGCGUCUCGCuCGAGCUUu -3' miRNA: 3'- guGCUUG-CUGCAGAGCGcGCUUGAAc -5' |
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| 12096 | 5' | -51.7 | NC_003309.1 | + | 40501 | 0.69 | 0.768222 |
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Target: 5'- uGCGGACGugGUCUggcCGCgGCGAGg--- -3' miRNA: 3'- gUGCUUGCugCAGA---GCG-CGCUUgaac -5' |
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| 12096 | 5' | -51.7 | NC_003309.1 | + | 46363 | 0.66 | 0.885975 |
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Target: 5'- cCGCGAGCGcCGcCUCGaucugcuuaCGCGGACg-- -3' miRNA: 3'- -GUGCUUGCuGCaGAGC---------GCGCUUGaac -5' |
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| 12096 | 5' | -51.7 | NC_003309.1 | + | 46794 | 0.66 | 0.900498 |
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Target: 5'- aCACGuacGCGugGcCgcuGCGCGAGCUg- -3' miRNA: 3'- -GUGCu--UGCugCaGag-CGCGCUUGAac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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