Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 121 | 3' | -56 | AC_000006.1 | + | 12373 | 0.66 | 0.554973 |
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Target: 5'- gCGGGCugUGGGGCgUGCaGGCgccCGUGGg -3' miRNA: 3'- aGUCCG--ACCUCG-GCG-CUGauuGCACC- -5' |
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| 121 | 3' | -56 | AC_000006.1 | + | 23624 | 0.68 | 0.41941 |
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Target: 5'- cCAGGCccUGGAGCaGCGgcgcaagcucauGCUGGcCGUGGu -3' miRNA: 3'- aGUCCG--ACCUCGgCGC------------UGAUU-GCACC- -5' |
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| 121 | 3' | -56 | AC_000006.1 | + | 20649 | 0.68 | 0.41941 |
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Target: 5'- ---cGCUGGAGCCGCucauGCUuagGugGUGGc -3' miRNA: 3'- agucCGACCUCGGCGc---UGA---UugCACC- -5' |
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| 121 | 3' | -56 | AC_000006.1 | + | 10880 | 0.69 | 0.372813 |
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Target: 5'- uUCGGGCgggcagGGAGCUGCG-CgagGGCcUGGa -3' miRNA: 3'- -AGUCCGa-----CCUCGGCGCuGa--UUGcACC- -5' |
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| 121 | 3' | -56 | AC_000006.1 | + | 23158 | 0.7 | 0.313735 |
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Target: 5'- gCGGGUUGGGcucgcagguGCCGUugGGCUGGCGUuGGg -3' miRNA: 3'- aGUCCGACCU---------CGGCG--CUGAUUGCA-CC- -5' |
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| 121 | 3' | -56 | AC_000006.1 | + | 16593 | 0.75 | 0.15183 |
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Target: 5'- gCuGGCUGGGcGCCuGCGGCUGGCGUa- -3' miRNA: 3'- aGuCCGACCU-CGG-CGCUGAUUGCAcc -5' |
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| 121 | 3' | -56 | AC_000006.1 | + | 10299 | 1.11 | 0.000383 |
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Target: 5'- aUCAGGCUGGAGCCGCGACUAACGUGGu -3' miRNA: 3'- -AGUCCGACCUCGGCGCUGAUUGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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