Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12103 | 3' | -53.4 | NC_003309.1 | + | 7830 | 0.66 | 0.7997 |
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Target: 5'- --aCGGcCGCCAGUcagCccGGCGAGCg -3' miRNA: 3'- cgaGCUaGCGGUCAaa-GcuCCGCUCG- -5' |
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| 12103 | 3' | -53.4 | NC_003309.1 | + | 10866 | 0.67 | 0.779942 |
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Target: 5'- cGCUCGcgCGCCug---CGuGGCaAGCg -3' miRNA: 3'- -CGAGCuaGCGGucaaaGCuCCGcUCG- -5' |
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| 12103 | 3' | -53.4 | NC_003309.1 | + | 22454 | 0.67 | 0.778937 |
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Target: 5'- cGCggCGAUCgcacgcuGCCGGcgUCGAGcGCGGcGCg -3' miRNA: 3'- -CGa-GCUAG-------CGGUCaaAGCUC-CGCU-CG- -5' |
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| 12103 | 3' | -53.4 | NC_003309.1 | + | 49831 | 0.68 | 0.692342 |
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Target: 5'- gGCUCGcUCGCCAGaaucaggaaCGAcacgugcagcgcuuGGCGAGUg -3' miRNA: 3'- -CGAGCuAGCGGUCaaa------GCU--------------CCGCUCG- -5' |
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| 12103 | 3' | -53.4 | NC_003309.1 | + | 12586 | 0.69 | 0.673682 |
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Target: 5'- cGCUCGAUC-UCGGcgauguacUCG-GGCGAGCc -3' miRNA: 3'- -CGAGCUAGcGGUCaa------AGCuCCGCUCG- -5' |
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| 12103 | 3' | -53.4 | NC_003309.1 | + | 49649 | 0.69 | 0.662648 |
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Target: 5'- aGCUCGA-CGCCc---UCGA-GCGGGCa -3' miRNA: 3'- -CGAGCUaGCGGucaaAGCUcCGCUCG- -5' |
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| 12103 | 3' | -53.4 | NC_003309.1 | + | 37033 | 0.69 | 0.640504 |
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Target: 5'- aGUcgCGAUCGCCaAGgg-CGAGGcCGaAGCg -3' miRNA: 3'- -CGa-GCUAGCGG-UCaaaGCUCC-GC-UCG- -5' |
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| 12103 | 3' | -53.4 | NC_003309.1 | + | 10397 | 0.8 | 0.172325 |
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Target: 5'- aGCUCGAUCGCCcug--CGcuuuGGCGAGCg -3' miRNA: 3'- -CGAGCUAGCGGucaaaGCu---CCGCUCG- -5' |
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| 12103 | 3' | -53.4 | NC_003309.1 | + | 1703 | 0.8 | 0.163116 |
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Target: 5'- aGCUUGcgCGCCAug-UCGAGcGCGAGCa -3' miRNA: 3'- -CGAGCuaGCGGUcaaAGCUC-CGCUCG- -5' |
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| 12103 | 3' | -53.4 | NC_003309.1 | + | 5179 | 1.14 | 0.000716 |
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Target: 5'- cGCUCGAUCGCCAGUUUCGAGGCGAGCu -3' miRNA: 3'- -CGAGCUAGCGGUCAAAGCUCCGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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