Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12105 | 5' | -61.7 | NC_003309.1 | + | 22145 | 0.69 | 0.266569 |
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Target: 5'- cGCgacACGCGCCCC--GCCAguCGCCcGCa -3' miRNA: 3'- -CGaa-UGCGCGGGGucCGGU--GCGGcCG- -5' |
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| 12105 | 5' | -61.7 | NC_003309.1 | + | 45955 | 0.69 | 0.266569 |
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Target: 5'- cGCgcaugACGCGCUUCGGGau-CGCCGGa -3' miRNA: 3'- -CGaa---UGCGCGGGGUCCgguGCGGCCg -5' |
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| 12105 | 5' | -61.7 | NC_003309.1 | + | 10866 | 0.69 | 0.260111 |
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Target: 5'- cGCUcGCGCGCCugCguGGCaagcgcgacauCACGCuCGGCu -3' miRNA: 3'- -CGAaUGCGCGG--GguCCG-----------GUGCG-GCCG- -5' |
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| 12105 | 5' | -61.7 | NC_003309.1 | + | 11993 | 0.69 | 0.25378 |
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Target: 5'- uGCUgcccGCGCGCggCAGccgcggcgcgcGCCGCGUCGGCg -3' miRNA: 3'- -CGAa---UGCGCGggGUC-----------CGGUGCGGCCG- -5' |
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| 12105 | 5' | -61.7 | NC_003309.1 | + | 28493 | 0.71 | 0.19226 |
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Target: 5'- ---aGCgGCGaCCCgCGcGCCACGCCGGCc -3' miRNA: 3'- cgaaUG-CGC-GGG-GUcCGGUGCGGCCG- -5' |
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| 12105 | 5' | -61.7 | NC_003309.1 | + | 10576 | 0.73 | 0.126218 |
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Target: 5'- cGCUUGCGcCGCCgCCucgacGGCaCGCGCuuCGGCa -3' miRNA: 3'- -CGAAUGC-GCGG-GGu----CCG-GUGCG--GCCG- -5' |
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| 12105 | 5' | -61.7 | NC_003309.1 | + | 540 | 0.73 | 0.122877 |
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Target: 5'- gGCUU-UGCGCCgaCCAGGCgcUGCCGGCu -3' miRNA: 3'- -CGAAuGCGCGG--GGUCCGguGCGGCCG- -5' |
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| 12105 | 5' | -61.7 | NC_003309.1 | + | 11523 | 0.86 | 0.014254 |
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Target: 5'- uGCUUGCGCGCCCUucugcgacgcgAGGCUcgccgugucgaucGCGCCGGCg -3' miRNA: 3'- -CGAAUGCGCGGGG-----------UCCGG-------------UGCGGCCG- -5' |
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| 12105 | 5' | -61.7 | NC_003309.1 | + | 6124 | 1.12 | 0.00015 |
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Target: 5'- cGCUUACGCGCCCCAGGCCACGCCGGCc -3' miRNA: 3'- -CGAAUGCGCGGGGUCCGGUGCGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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