Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12107 | 3' | -55.3 | NC_003309.1 | + | 50196 | 0.66 | 0.688733 |
Target: 5'- cGAGCgCGagguucgaaaUGCCGGGGAacGACG-GCUCCa -3' miRNA: 3'- -CUUG-GC----------GCGGCCUCUa-CUGUaCGAGG- -5' |
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12107 | 3' | -55.3 | NC_003309.1 | + | 43438 | 0.7 | 0.443316 |
Target: 5'- cAGCCGCGCCGcGAGAuugaacagcaccUGuGCGUGCUg- -3' miRNA: 3'- cUUGGCGCGGC-CUCU------------AC-UGUACGAgg -5' |
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12107 | 3' | -55.3 | NC_003309.1 | + | 35094 | 0.66 | 0.710386 |
Target: 5'- cGAGCuCGCuGUCGGcGAUGuuGUGUUCUa -3' miRNA: 3'- -CUUG-GCG-CGGCCuCUACugUACGAGG- -5' |
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12107 | 3' | -55.3 | NC_003309.1 | + | 22054 | 0.69 | 0.546371 |
Target: 5'- -cACCGcCGCCGcGAGcUGAUAUGUgcgCCc -3' miRNA: 3'- cuUGGC-GCGGC-CUCuACUGUACGa--GG- -5' |
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12107 | 3' | -55.3 | NC_003309.1 | + | 13052 | 0.73 | 0.334741 |
Target: 5'- aAGCUGCGCCGG-GAUacugucgcucgugGACGUGC-CCg -3' miRNA: 3'- cUUGGCGCGGCCuCUA-------------CUGUACGaGG- -5' |
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12107 | 3' | -55.3 | NC_003309.1 | + | 11054 | 0.66 | 0.688733 |
Target: 5'- cGGCCGCGCCG-AGugcGAcCGUGC-CCa -3' miRNA: 3'- cUUGGCGCGGCcUCua-CU-GUACGaGG- -5' |
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12107 | 3' | -55.3 | NC_003309.1 | + | 7101 | 1.12 | 0.000612 |
Target: 5'- cGAACCGCGCCGGAGAUGACAUGCUCCu -3' miRNA: 3'- -CUUGGCGCGGCCUCUACUGUACGAGG- -5' |
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12107 | 3' | -55.3 | NC_003309.1 | + | 1196 | 0.67 | 0.655871 |
Target: 5'- -cGCCGuCGCCuGGGuugccGAUGAUcgGCUCa -3' miRNA: 3'- cuUGGC-GCGG-CCU-----CUACUGuaCGAGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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