miRNA: 3'- -GUCCuuag-CUUGUAGCCggGUGCCAG- -5'
miRNA: 3'- guCCuuAGC--UUGUAGC-CGGGuGCCaG- -5'
miRNA: 3'- -GUCCUUAGCUUGUAGCCGGGUGCCAG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12107 | 5' | -54.3 | NC_003309.1 | + | 39696 | 0.68 | 0.616379 |
|
Target: 5'- cCGGGcggaaGAACGUCGGaaUACGGUCg -3' miRNA: 3'- -GUCCuuag-CUUGUAGCCggGUGCCAG- -5' |
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| 12107 | 5' | -54.3 | NC_003309.1 | + | 26844 | 0.69 | 0.572241 |
|
Target: 5'- -cGGggUCGccGACAUCGaGCCC-CGG-Ca -3' miRNA: 3'- guCCuuAGC--UUGUAGC-CGGGuGCCaG- -5' |
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| 12107 | 5' | -54.3 | NC_003309.1 | + | 7067 | 1.1 | 0.00108 |
|
Target: 5'- uCAGGAAUCGAACAUCGGCCCACGGUCg -3' miRNA: 3'- -GUCCUUAGCUUGUAGCCGGGUGCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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