miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1211 3' -56.6 NC_001132.2 + 126191 0.66 0.913085
Target:  5'- cUUCGGGaGUugGgGUAgGGGCGGuCGUc -3'
miRNA:   3'- -AGGCCUgUAugCgCAUgCCCGCU-GCA- -5'
1211 3' -56.6 NC_001132.2 + 149579 0.66 0.911339
Target:  5'- aUUCGGAUAuauaccuaaccauUACGUGUcuggaaccggaGGGCGACGUg -3'
miRNA:   3'- -AGGCCUGU-------------AUGCGCAug---------CCCGCUGCA- -5'
1211 3' -56.6 NC_001132.2 + 30094 0.67 0.870065
Target:  5'- aCCGGACG-ACGuCGUACcuagucuguugcaauGGGCGAgCGa -3'
miRNA:   3'- aGGCCUGUaUGC-GCAUG---------------CCCGCU-GCa -5'
1211 3' -56.6 NC_001132.2 + 27488 0.67 0.867158
Target:  5'- -aCGaGACG-ACGCGUACGGGUauACGUa -3'
miRNA:   3'- agGC-CUGUaUGCGCAUGCCCGc-UGCA- -5'
1211 3' -56.6 NC_001132.2 + 2400 1.08 0.003719
Target:  5'- cUCCGGACAUACGCGUACGGGCGACGUg -3'
miRNA:   3'- -AGGCCUGUAUGCGCAUGCCCGCUGCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.