Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12110 | 3' | -53.5 | NC_003309.1 | + | 29584 | 0.66 | 0.796347 |
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Target: 5'- cGGCGU-GCGGGUcGAUuucaaGAUCACGCcCGa -3' miRNA: 3'- -CCGCAgUGCCUA-CUG-----CUAGUGCGaGU- -5' |
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| 12110 | 3' | -53.5 | NC_003309.1 | + | 44910 | 0.67 | 0.786521 |
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Target: 5'- gGGCGUCcaGCGGuacAUGAacUCGCGCUUg -3' miRNA: 3'- -CCGCAG--UGCCuacUGCU--AGUGCGAGu -5' |
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| 12110 | 3' | -53.5 | NC_003309.1 | + | 27938 | 0.67 | 0.776533 |
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Target: 5'- uGCGUCGCGGcgGGCa--CGCGCa-- -3' miRNA: 3'- cCGCAGUGCCuaCUGcuaGUGCGagu -5' |
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| 12110 | 3' | -53.5 | NC_003309.1 | + | 23590 | 0.67 | 0.756116 |
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Target: 5'- uGGuCGUUGaGGcUGACGAUCGCGCg-- -3' miRNA: 3'- -CC-GCAGUgCCuACUGCUAGUGCGagu -5' |
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| 12110 | 3' | -53.5 | NC_003309.1 | + | 10679 | 0.68 | 0.70304 |
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Target: 5'- cGGCGgccgucUCGCGGgcGAUGAUCGcCGCg-- -3' miRNA: 3'- -CCGC------AGUGCCuaCUGCUAGU-GCGagu -5' |
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| 12110 | 3' | -53.5 | NC_003309.1 | + | 26392 | 0.68 | 0.70304 |
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Target: 5'- cGGCaaccgccgCGCGGAcGGCGGgaucgaaugCACGCUCAa -3' miRNA: 3'- -CCGca------GUGCCUaCUGCUa--------GUGCGAGU- -5' |
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| 12110 | 3' | -53.5 | NC_003309.1 | + | 4663 | 0.68 | 0.692169 |
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Target: 5'- cGGCGcgcauUCGCGccuUGACGAUCG-GCUCGg -3' miRNA: 3'- -CCGC-----AGUGCcu-ACUGCUAGUgCGAGU- -5' |
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| 12110 | 3' | -53.5 | NC_003309.1 | + | 33314 | 0.73 | 0.405582 |
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Target: 5'- gGGCGUCGCcGGUGACGAaugggucaaggaCugGCUCGa -3' miRNA: 3'- -CCGCAGUGcCUACUGCUa-----------GugCGAGU- -5' |
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| 12110 | 3' | -53.5 | NC_003309.1 | + | 7702 | 1.11 | 0.001243 |
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Target: 5'- aGGCGUCACGGAUGACGAUCACGCUCAc -3' miRNA: 3'- -CCGCAGUGCCUACUGCUAGUGCGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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