Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12111 | 5' | -54.4 | NC_003309.1 | + | 7873 | 1.11 | 0.000942 |
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Target: 5'- cGGUGAACGUCGGCGCGUAGCAGUCGAu -3' miRNA: 3'- -CCACUUGCAGCCGCGCAUCGUCAGCU- -5' |
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| 12111 | 5' | -54.4 | NC_003309.1 | + | 37290 | 0.74 | 0.322565 |
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Target: 5'- -cUGAAucuCGcCGGCGCGUcgauGGCGGUCGAg -3' miRNA: 3'- ccACUU---GCaGCCGCGCA----UCGUCAGCU- -5' |
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| 12111 | 5' | -54.4 | NC_003309.1 | + | 36774 | 0.74 | 0.330505 |
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Target: 5'- --cGGACGcUCGGCG-GUGGCGGUUGGg -3' miRNA: 3'- ccaCUUGC-AGCCGCgCAUCGUCAGCU- -5' |
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| 12111 | 5' | -54.4 | NC_003309.1 | + | 6005 | 0.69 | 0.62532 |
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Target: 5'- uGUGGcCGUCGGCGUccuuguagGUGGCuuccuugcuguAGUCGAu -3' miRNA: 3'- cCACUuGCAGCCGCG--------CAUCG-----------UCAGCU- -5' |
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| 12111 | 5' | -54.4 | NC_003309.1 | + | 46665 | 0.69 | 0.62532 |
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Target: 5'- aGGUGAcCGggcCGGCGCGUAaugcGCAG-CGc -3' miRNA: 3'- -CCACUuGCa--GCCGCGCAU----CGUCaGCu -5' |
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| 12111 | 5' | -54.4 | NC_003309.1 | + | 15209 | 0.66 | 0.77472 |
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Target: 5'- --aGcACGUCGGCGCcgauuuccGUA-CAGUCGAc -3' miRNA: 3'- ccaCuUGCAGCCGCG--------CAUcGUCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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