miRNA: 3'- gaGuGUGCGUGCGacAUGCAGCGGcu -5'
miRNA: 3'- gaGUGUGCGUGCGacauGCAGCgGCU- -5'
miRNA: 3'- gAGU-GUGCGUGCGa----CAUGc-----AGCGGCu -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12113 | 3' | -55.5 | NC_003309.1 | + | 39277 | 0.69 | 0.500377 |
|
Target: 5'- -aCuCGCGCAUGCguUGCGUCGCCa- -3' miRNA: 3'- gaGuGUGCGUGCGacAUGCAGCGGcu -5' |
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| 12113 | 3' | -55.5 | NC_003309.1 | + | 43539 | 0.67 | 0.661998 |
|
Target: 5'- -gCACGCGCACGC----CGUCGgCGGc -3' miRNA: 3'- gaGUGUGCGUGCGacauGCAGCgGCU- -5' |
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| 12113 | 3' | -55.5 | NC_003309.1 | + | 47921 | 0.66 | 0.683767 |
|
Target: 5'- gUCGuCGCGCACGCgugccGUGCcaaccaUCGCCGu -3' miRNA: 3'- gAGU-GUGCGUGCGa----CAUGc-----AGCGGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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