Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12114 | 5' | -54.4 | NC_003309.1 | + | 7618 | 0.66 | 0.791105 |
Target: 5'- cUCGGCc--UCGCCGAu--CGACGCa -3' miRNA: 3'- aGGCCGcaaAGUGGCUucuGCUGCGc -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 47733 | 0.66 | 0.791105 |
Target: 5'- gCCGGC----CGCCuu-GACGACGCGc -3' miRNA: 3'- aGGCCGcaaaGUGGcuuCUGCUGCGC- -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 35705 | 0.66 | 0.781297 |
Target: 5'- -aCGGCuucGUUUCGCuCGAAGGuCuGCGCGa -3' miRNA: 3'- agGCCG---CAAAGUG-GCUUCU-GcUGCGC- -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 22486 | 0.66 | 0.781297 |
Target: 5'- -aCGGCGgc-CACCGccGACGcggcggcaucguGCGCGg -3' miRNA: 3'- agGCCGCaaaGUGGCuuCUGC------------UGCGC- -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 25554 | 0.66 | 0.781297 |
Target: 5'- gUCgGGUGUagCggGCCGAAGACGcACGgGc -3' miRNA: 3'- -AGgCCGCAaaG--UGGCUUCUGC-UGCgC- -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 19073 | 0.66 | 0.771336 |
Target: 5'- -aCGGCGguggcugUACCGAAGGCGGaacaauCGUGa -3' miRNA: 3'- agGCCGCaaa----GUGGCUUCUGCU------GCGC- -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 13982 | 0.66 | 0.761232 |
Target: 5'- aUUCGGCcg--CACCGA---CGACGCGg -3' miRNA: 3'- -AGGCCGcaaaGUGGCUucuGCUGCGC- -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 27105 | 0.66 | 0.761232 |
Target: 5'- gCCGcaGUGccUUAUCGAGGACGACGUa -3' miRNA: 3'- aGGC--CGCaaAGUGGCUUCUGCUGCGc -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 12056 | 0.66 | 0.760215 |
Target: 5'- -gCGGCGUugguggauacgcgUgacgucugCGCCGcAGugGACGCGa -3' miRNA: 3'- agGCCGCA-------------Aa-------GUGGCuUCugCUGCGC- -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 15596 | 0.67 | 0.740645 |
Target: 5'- cUUCGGCaGUUgcuggcCACCGAAGucgaGCGGCGaCGa -3' miRNA: 3'- -AGGCCG-CAAa-----GUGGCUUC----UGCUGC-GC- -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 5402 | 0.68 | 0.654861 |
Target: 5'- aUCGGUGUcagCACCGAuguagccGACGAuCGCGc -3' miRNA: 3'- aGGCCGCAaa-GUGGCUu------CUGCU-GCGC- -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 18730 | 0.7 | 0.535589 |
Target: 5'- gUCGaGCGUgaucgucUCGCCGGcuGCGGCGCGg -3' miRNA: 3'- aGGC-CGCAa------AGUGGCUucUGCUGCGC- -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 16019 | 0.7 | 0.525034 |
Target: 5'- -gCGGCGUagUCAUCGGAuGCGAgCGCGa -3' miRNA: 3'- agGCCGCAa-AGUGGCUUcUGCU-GCGC- -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 25250 | 0.72 | 0.443937 |
Target: 5'- gUCCGGCccucGUgaCAUCGAcugcgGGACGACGCu -3' miRNA: 3'- -AGGCCG----CAaaGUGGCU-----UCUGCUGCGc -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 7760 | 0.75 | 0.290789 |
Target: 5'- gCCGGCGccUUCGCCGGu-GCGACGCc -3' miRNA: 3'- aGGCCGCa-AAGUGGCUucUGCUGCGc -5' |
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12114 | 5' | -54.4 | NC_003309.1 | + | 8865 | 1.1 | 0.001244 |
Target: 5'- gUCCGGCGUUUCACCGAAGACGACGCGa -3' miRNA: 3'- -AGGCCGCAAAGUGGCUUCUGCUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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