Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12115 | 5' | -54.2 | NC_003309.1 | + | 9146 | 1.11 | 0.000856 |
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Target: 5'- aUCAUCUGCAACCGGCGAUUCCGCGUCa -3' miRNA: 3'- -AGUAGACGUUGGCCGCUAAGGCGCAG- -5' |
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| 12115 | 5' | -54.2 | NC_003309.1 | + | 44223 | 0.74 | 0.298186 |
|
Target: 5'- cCGUCUGcCAACCGGCGA--CCGCu-- -3' miRNA: 3'- aGUAGAC-GUUGGCCGCUaaGGCGcag -5' |
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| 12115 | 5' | -54.2 | NC_003309.1 | + | 41701 | 0.69 | 0.606596 |
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Target: 5'- -aAUUUGacaAGCCGGCcgcgcUCCGCGUCg -3' miRNA: 3'- agUAGACg--UUGGCCGcua--AGGCGCAG- -5' |
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| 12115 | 5' | -54.2 | NC_003309.1 | + | 46446 | 0.69 | 0.606596 |
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Target: 5'- cUCAUUcgGCGGCCuccuguGCGcuUUCCGCGUCg -3' miRNA: 3'- -AGUAGa-CGUUGGc-----CGCu-AAGGCGCAG- -5' |
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| 12115 | 5' | -54.2 | NC_003309.1 | + | 8559 | 0.68 | 0.651117 |
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Target: 5'- cUAUCUGCGAUCGGgGAguuacgCCGCc-- -3' miRNA: 3'- aGUAGACGUUGGCCgCUaa----GGCGcag -5' |
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| 12115 | 5' | -54.2 | NC_003309.1 | + | 48474 | 0.68 | 0.639987 |
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Target: 5'- aUCGUCagcccgcgGCGGCCGGCc-UUCCGCuUCa -3' miRNA: 3'- -AGUAGa-------CGUUGGCCGcuAAGGCGcAG- -5' |
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| 12115 | 5' | -54.2 | NC_003309.1 | + | 47021 | 0.67 | 0.71713 |
|
Target: 5'- gCA-CUGCAcgCGGCGAUUggCGCGUCu -3' miRNA: 3'- aGUaGACGUugGCCGCUAAg-GCGCAG- -5' |
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| 12115 | 5' | -54.2 | NC_003309.1 | + | 11988 | 0.67 | 0.706275 |
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Target: 5'- cCcgCgcgcgGCAGCCgcGGCGcgcgCCGCGUCg -3' miRNA: 3'- aGuaGa----CGUUGG--CCGCuaa-GGCGCAG- -5' |
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| 12115 | 5' | -54.2 | NC_003309.1 | + | 31921 | 0.66 | 0.727899 |
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Target: 5'- -----cGCGGCCGGCGccagCGCGUCu -3' miRNA: 3'- aguagaCGUUGGCCGCuaagGCGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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