Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12116 | 5' | -56.2 | NC_003309.1 | + | 10489 | 0.66 | 0.626103 |
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Target: 5'- -cGCGaGGCuuuCGCG-CAUCGCGCg -3' miRNA: 3'- gaCGCaCCGuucGCGCuGUAGUGCGa -5' |
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| 12116 | 5' | -56.2 | NC_003309.1 | + | 10730 | 0.66 | 0.604014 |
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Target: 5'- gCUGC-UGcGCGAGCGCGAgcgaagccuCggCGCGCUc -3' miRNA: 3'- -GACGcAC-CGUUCGCGCU---------GuaGUGCGA- -5' |
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| 12116 | 5' | -56.2 | NC_003309.1 | + | 10386 | 0.67 | 0.582022 |
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Target: 5'- cCUGCGcuuUGGCGAGCGCcGCcu-GCGCc -3' miRNA: 3'- -GACGC---ACCGUUCGCGcUGuagUGCGa -5' |
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| 12116 | 5' | -56.2 | NC_003309.1 | + | 41362 | 0.67 | 0.560198 |
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Target: 5'- -cGCGUGGUuGGCuCGACAUCGaucaugaGCg -3' miRNA: 3'- gaCGCACCGuUCGcGCUGUAGUg------CGa -5' |
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| 12116 | 5' | -56.2 | NC_003309.1 | + | 11984 | 0.68 | 0.486018 |
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Target: 5'- -cGCGcGGCAGcCGCGGCG-CGCGCc -3' miRNA: 3'- gaCGCaCCGUUcGCGCUGUaGUGCGa -5' |
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| 12116 | 5' | -56.2 | NC_003309.1 | + | 46787 | 0.7 | 0.380092 |
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Target: 5'- -cGCGUGGCcgcuGCGCGAgcUGUCACcGCa -3' miRNA: 3'- gaCGCACCGuu--CGCGCU--GUAGUG-CGa -5' |
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| 12116 | 5' | -56.2 | NC_003309.1 | + | 39264 | 0.71 | 0.326784 |
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Target: 5'- uUGCGUcGCcAGCGCGcagcccgcggaucaGCAUCGCGCa -3' miRNA: 3'- gACGCAcCGuUCGCGC--------------UGUAGUGCGa -5' |
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| 12116 | 5' | -56.2 | NC_003309.1 | + | 22469 | 0.72 | 0.313383 |
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Target: 5'- -cGCGgcGGCAucguGCGCGGCGaucgCACGCUg -3' miRNA: 3'- gaCGCa-CCGUu---CGCGCUGUa---GUGCGA- -5' |
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| 12116 | 5' | -56.2 | NC_003309.1 | + | 10856 | 1.07 | 0.000949 |
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Target: 5'- cCUGCGUGGCAAGCGCGACAUCACGCUc -3' miRNA: 3'- -GACGCACCGUUCGCGCUGUAGUGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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