Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12117 | 3' | -59.4 | NC_003309.1 | + | 19875 | 0.66 | 0.431607 |
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Target: 5'- cGCGACCGUGUcgaccuuuuucgcgaCCGCcauGUCGCCc--- -3' miRNA: 3'- aCGCUGGCACG---------------GGUGu--CAGCGGucuu -5' |
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| 12117 | 3' | -59.4 | NC_003309.1 | + | 48026 | 0.67 | 0.418424 |
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Target: 5'- aGUGGCCG-GUCga-GGUCGCCGGAu -3' miRNA: 3'- aCGCUGGCaCGGgugUCAGCGGUCUu -5' |
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| 12117 | 3' | -59.4 | NC_003309.1 | + | 22145 | 0.67 | 0.373411 |
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Target: 5'- cGCGACaCGcGCCCcgcCAGUCGCCc--- -3' miRNA: 3'- aCGCUG-GCaCGGGu--GUCAGCGGucuu -5' |
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| 12117 | 3' | -59.4 | NC_003309.1 | + | 11263 | 0.67 | 0.373411 |
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Target: 5'- uUGCG-CC--GCCCGCA-UCGCCAGAc -3' miRNA: 3'- -ACGCuGGcaCGGGUGUcAGCGGUCUu -5' |
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| 12117 | 3' | -59.4 | NC_003309.1 | + | 49997 | 0.68 | 0.323898 |
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Target: 5'- aGCGcACUGUGCCCGCAcuucCGCCAu-- -3' miRNA: 3'- aCGC-UGGCACGGGUGUca--GCGGUcuu -5' |
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| 12117 | 3' | -59.4 | NC_003309.1 | + | 49940 | 0.7 | 0.272525 |
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Target: 5'- gGCGGCCGaGCCgGuCAGUUGCCcGAGc -3' miRNA: 3'- aCGCUGGCaCGGgU-GUCAGCGGuCUU- -5' |
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| 12117 | 3' | -59.4 | NC_003309.1 | + | 7117 | 0.74 | 0.137053 |
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Target: 5'- cGCGACCGuUGCaCCACGaaccgCGCCGGAGa -3' miRNA: 3'- aCGCUGGC-ACG-GGUGUca---GCGGUCUU- -5' |
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| 12117 | 3' | -59.4 | NC_003309.1 | + | 11042 | 1.06 | 0.000565 |
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Target: 5'- gUGCGACCGUGCCCACAGUCGCCAGAAu -3' miRNA: 3'- -ACGCUGGCACGGGUGUCAGCGGUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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