Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12118 | 5' | -55 | NC_003309.1 | + | 24029 | 0.68 | 0.561309 |
Target: 5'- --cGACACg--GCuccaCCGCCCGuCGUCGa -3' miRNA: 3'- guaCUGUGaaaCGc---GGCGGGC-GUAGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 11273 | 1.08 | 0.001182 |
Target: 5'- cCAUGACACUUUGCGCCGCCCGCAUCGc -3' miRNA: 3'- -GUACUGUGAAACGCGGCGGGCGUAGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 48837 | 0.72 | 0.354228 |
Target: 5'- -cUGACGCUUcaGCGUCGCCguuCGCGUCa -3' miRNA: 3'- guACUGUGAAa-CGCGGCGG---GCGUAGc -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 10306 | 0.72 | 0.354228 |
Target: 5'- ---cACGCUgu-CGCCGCCUGCGUCGc -3' miRNA: 3'- guacUGUGAaacGCGGCGGGCGUAGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 25390 | 0.71 | 0.426884 |
Target: 5'- uCGUGcCACUgcgUGUaGCCGUCgGCGUCGg -3' miRNA: 3'- -GUACuGUGAa--ACG-CGGCGGgCGUAGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 27746 | 0.71 | 0.426884 |
Target: 5'- --aGACGac--GCGCCGCCCggcGCAUCGc -3' miRNA: 3'- guaCUGUgaaaCGCGGCGGG---CGUAGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 10581 | 0.7 | 0.446385 |
Target: 5'- aCAUG-CGC-UUGCGCCGC-CGCcUCGa -3' miRNA: 3'- -GUACuGUGaAACGCGGCGgGCGuAGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 19890 | 0.7 | 0.497191 |
Target: 5'- --cGAuCGCUgcUGCGCCGCgaCCGUGUCGa -3' miRNA: 3'- guaCU-GUGAa-ACGCGGCG--GGCGUAGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 22143 | 0.69 | 0.528893 |
Target: 5'- --cGACAC---GCGCCccgccagucGCCCGCAUUGa -3' miRNA: 3'- guaCUGUGaaaCGCGG---------CGGGCGUAGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 10384 | 0.69 | 0.528893 |
Target: 5'- ---uGCGCUUUggcgaGCGCCGCCUGCGcCGc -3' miRNA: 3'- guacUGUGAAA-----CGCGGCGGGCGUaGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 10071 | 0.65 | 0.736874 |
Target: 5'- --cGAacaGCguauccGUGCCGCCCGCcgCGc -3' miRNA: 3'- guaCUg--UGaaa---CGCGGCGGGCGuaGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 53052 | 0.68 | 0.583222 |
Target: 5'- gCAUGACGa-UUGC-CgCGCaCCGCGUCGg -3' miRNA: 3'- -GUACUGUgaAACGcG-GCG-GGCGUAGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 44133 | 0.68 | 0.594245 |
Target: 5'- gGUGAUGCU---CGUCGCCCGCcgCa -3' miRNA: 3'- gUACUGUGAaacGCGGCGGGCGuaGc -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 22394 | 0.68 | 0.616379 |
Target: 5'- gCAUGcCGCUgcGCGUcgaaCGCCCGCG-CGg -3' miRNA: 3'- -GUACuGUGAaaCGCG----GCGGGCGUaGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 33004 | 0.67 | 0.64966 |
Target: 5'- --cGACACU---UGCCGCauCCGCAUCu -3' miRNA: 3'- guaCUGUGAaacGCGGCG--GGCGUAGc -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 12039 | 0.67 | 0.64966 |
Target: 5'- gCGUGACGuc-UGCGCCGCaguggaCGCgAUCGu -3' miRNA: 3'- -GUACUGUgaaACGCGGCGg-----GCG-UAGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 1986 | 0.67 | 0.660735 |
Target: 5'- --cGACAgguucgUUGCGCCGUgCGgGUCGa -3' miRNA: 3'- guaCUGUga----AACGCGGCGgGCgUAGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 20023 | 0.66 | 0.682792 |
Target: 5'- --cGACGgUUUGCaCCGCCgcCGCGUUGu -3' miRNA: 3'- guaCUGUgAAACGcGGCGG--GCGUAGC- -5' |
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12118 | 5' | -55 | NC_003309.1 | + | 52383 | 0.66 | 0.704652 |
Target: 5'- gCAUGACGaagaUGCGCUcgccgcagcaGCCgGCAUCa -3' miRNA: 3'- -GUACUGUgaa-ACGCGG----------CGGgCGUAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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